Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis
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Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 9, Pages: 22-24(2010)
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Published:2010
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ZHU Xiao-yue, Liu Hong, HAO Yu, et al. Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 22-24.
DOI:
ZHU Xiao-yue, Liu Hong, HAO Yu, et al. Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 22-24.DOI:
Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis
Objective: To study the extraction and purification technology of total alkaloids in Folium Nelumbinis. Method: The total alkaloids were obtained by impregnation
concentration
Soxhlet extraction and alkaline separation. Acid dye colorimetry was used to determine total alkaloids in Folium Nelumbinis. Result: Folium Nelumbinis was soaked in 1% HCl/70% ethanol for 36 h 3 times. After neutralization and concentration
Soxhlet extraction was carried out for 3 hours with ethyl acetate. Then the concentrate was extracted successively by acidic water at pH 1 and ethyl acetate. 40% NaOH was used for alkaline separation. The content of total alkaloids was above 90%. Conclusion: This extraction and purification technology was convenient
feasible
and reproducible
that would provide basic for the research and development of Folium Nelumbinis.
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