Determination of Oleanolic Acid in dandelion by RP-HPLC
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Determination of Oleanolic Acid in dandelion by RP-HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 5, Pages: 52-54(2010)
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Published:2010
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LI Xi-feng, HAO Zhe, QIU Tian-bao, et al. Determination of Oleanolic Acid in dandelion by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 52-54.
DOI:
LI Xi-feng, HAO Zhe, QIU Tian-bao, et al. Determination of Oleanolic Acid in dandelion by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 52-54.DOI:
Determination of Oleanolic Acid in dandelion by RP-HPLC
流速1.0 mL ·min-1检测波长210 nm。 结果 :齐墩果酸在0.872~7.848 μg范围内峰面积与进样量有良好的线性关系
回归方程为:Y=6.937 3X-1.989 4
r=0.999 9
平均回收率为98.25%
RSD为1.54%。 结论 :该法简便快速、准确
重复性好
有助于蒲公英质量控制方法的研究。
Abstract
Objective: To establish the determination method for oleanolic acid in dandelion by HPLC. Method: HPLC was carried out on Hypersil ODS2 C18(4.6 mm×250 mm
5 μm)at 30 ℃ using methanol-0.5% H3PO4 (pH=3.5)(90 ∶10) as mobile phase with a flow rate of 1.0 mL ·min-1 and detection wavelength was at 210 nm. Result :The oleanolic acid was linear within the range of 0.872~7.848 μg. The average recovery was 98.25% and RSD was 1.54%. Conclusion :This method is useful for quality control of dandelion.
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Related Author
LI Xi-feng
HAO Zhe
QIU Tian-bao
YU Yun-hui
DU Yun-feng
LI Xi-feng
HU Ya-nan
SHI Yang
Related Institution
Henan College of TCM
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine
Jilin Ginseng Academy,Changchun University of Chinese Medicine
Chengdu University of Traditional Chinese Medicine