Determination of Homocysteine Level in Rats' Plasma by HPLC-ECD
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Determination of Homocysteine Level in Rats' Plasma by HPLC-ECD
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 3, Pages: 116-118(2010)
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Published:2010
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SONG Wen-ting, LI Lei, LIN Cheng-ren, et al. Determination of Homocysteine Level in Rats' Plasma by HPLC-ECD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 116-118.
DOI:
SONG Wen-ting, LI Lei, LIN Cheng-ren, et al. Determination of Homocysteine Level in Rats' Plasma by HPLC-ECD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 116-118.DOI:
Determination of Homocysteine Level in Rats' Plasma by HPLC-ECD
Objective: To establish a method for assay of homocysteine in rats plasma with High Performance Liquid Chromatography and Electrochemical Detector. Methods: Plasma homocysteine was reduced by dithiothreitol
separated on reverse phase ODS2 column with 10% methanol mobile phase and detected on ESA 5600 electrochemical detector. Results: The method showed excellent linearity over the concentration range 0.1~100 μmol·L-1 of Hcy (r=0.999 3
n=7). The recoveries of analytes were from 96.79 %~104.40 %. The precisions
and the stability of the analytes meet the requirements. Conclusion: The established method show high sensitivity and good reproducibility
which make it suitable to determine Hcy level in rats plasma.
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Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
College of Traditional Chinese Medicine(TCM), Yunnan University of Chinese Medicine
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, The First Affiliated Hospital of Xinjiang Medical University