Objective: To compare depression effects of rhubarb aglycone associated thrombolysis using at different time windows through artery on neurocyte apoptosis and its correlated controlling gene in rats with thrombus-occluded cerebral ischemia. Method: Rats were randomly divided into sham-operated

model

urokinase thrombolysis

rhubarb aglycone and associated groups. Rat model of thrombus-occluded cerebral ischemic was duplicated through the occlusion of middle cerebral artery by using the embolus of rat autologous blood blot and being inserted with nylon thread. At the time of 3

6

9 h after the operation of cerebral ischemia

rats were underwent treatment of thrombolysis by using urokinase through the pipe in internal carotid artery. At 24 h after urokinase thrombolysis

rats brain were taken

and then brain pathological changes were observed. Immunohistochemical method was used to detect the brain neurocyte apoptosis (TUNEL cell number)

and then the expression of Bax

caspase-3 and Bcl-2 were measured in the study. Result: Compared with sham-operated group

pathological changes were obvious

TUNEL cell numbers and expression of Bax

Caspase-3 increased in model group

while expression of Bcl-2 decreased. TUNEL cell numbers in each administration group decreased and expression of Bax

caspase-3 in 6

9 h groups of administration attenuated

while expression of Bcl-2 up regulated in 6 h groups

these changes appeared more significant in 3 h group than that in 9 h group. In comparison with each simple administration group

TUNEL cell numbers in association group reduced

and expression of Bax

caspase-3 in 6

9 h group attenuated

and expression of Bcl-2 up regulated. Conclusion: Up regulation of expression in promoting apoptosis and down regulation of expression in inhibiting apoptosis could be caused by injury of cerebral ischemia and the changes became more obvious following the prolonging of ischemia time. Rhubarb aglycone and thrombolysis could inhibit apoptosis

but effects of their association appeared more significant. Association of rhubarb aglycone and thrombolysis could inhibit apoptosis by up regulating expression of Bax and caspase-3

as well as down regulating expression of Bcl-2.