Effect of Taxol Combining with Cephalomannine on Apoptosis of Liver Cancer Cell HepG2

LIU Tong-xiang; ZHANG Yang-ping; XU Yu; HUANG Huo-qiang; XU Si-fan

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Effect of Taxol Combining with Cephalomannine on Apoptosis of Liver Cancer Cell HepG2

更新时间：2024-01-04

- Effect of Taxol Combining with Cephalomannine on Apoptosis of Liver Cancer Cell HepG2
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 9, Pages: 115-119(2010)
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- Published：2010
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LIU Tong-xiang, ZHANG Yang-ping, XU Yu, et al. Effect of Taxol Combining with Cephalomannine on Apoptosis of Liver Cancer Cell HepG2[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 115-119.
DOI：

LIU Tong-xiang, ZHANG Yang-ping, XU Yu, et al. Effect of Taxol Combining with Cephalomannine on Apoptosis of Liver Cancer Cell HepG2[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 115-119. DOI：

摘要

目的 :以HepG2 细胞作为体外模型

研究紫杉醇与三尖杉宁碱联合作用抑制肿瘤细胞增殖及凋亡双重作用。方法 :MTT法检测不同浓度紫杉醇、三尖杉宁碱单药及不同联合给药方案对HepG2 细胞的生长抑制作用;以PI染色法流式细胞仪分析不同给药方案对HepG2细胞周期分布的影响

Annexin V-FITC／PI双染色法分析不同联合给药方案对HepG2细胞凋亡的影响。结果 : MTT法证实不同给药方案对HepG2细胞抑制强度不同

紫杉醇和三尖杉宁碱同时给药无明显协同作用;先给予三尖杉宁碱24 h再给予紫杉醇和先给予紫杉醇24 h再给予三尖杉宁碱可见明显协同作用

先给予紫杉醇24 h再给予三尖杉宁碱协同作用更加明显

流式细胞仪检测结果显示

紫杉醇与三尖杉宁碱联合用药较二者单独用药可诱导更多HepG2细胞凋亡。结论 : 紫杉醇与三尖杉宁碱联合应用可协同抑制HepG2 细胞增殖

诱导肿瘤细胞凋亡。

Abstract

Objective: To investigate the function of taxol combining with cephalomannine on apoptosis of liver cancer cell HepG2. Method: The inhibitory rates of taxol and cephalomannine alone or different combinations of taxol and cephalomannine on HepG2 cells were examined by MTT assay.The HepG2 cells of control group

cephalomannine group

taxol group and the combination group were stained by PI and Annexin V-FITC／PI

the cell cycle distribution and the apoptosis rates were detected by flow cytometry. Result: MTT assay showed different combination schemes had different inhibitory effects. The synergistic effects was seen in Scheme2 (HepG2 cells were treated with cephalomannin firstly and successively treated with taxol) and Scheme3(HepG2 cells were treated with taxol firstly and successively treated with cephalomannin). Conclusion: Taxol combining with cephalomannine can definitely inhibit the growth of liver cancer cell HepG2 in vitro

and induce apoptosis for the cancer cell.

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