Extraction and Assaying of Safflower Polysaccharide

ZHANG Xiao-li; LI Yu-ting; WANG Ya-xian; SHI Xue-kui; LI Fu-juan; GUI Jin-qiu

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Extraction and Assaying of Safflower Polysaccharide

更新时间：2024-01-04

- Extraction and Assaying of Safflower Polysaccharide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 7, Pages: 19-21(2010)
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- Published：2010
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ZHANG Xiao-li, LI Yu-ting, WANG Ya-xian, et al. Extraction and Assaying of Safflower Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 19-21.
DOI：

ZHANG Xiao-li, LI Yu-ting, WANG Ya-xian, et al. Extraction and Assaying of Safflower Polysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 19-21. DOI：

摘要

目的: 研究红花多糖的提取及含量测定

为开展红花多糖的抗肿瘤作用机制提供理想药物。方法: 采用水提醇沉法从红花药材中提取并分离出红花粗多糖

用Sevage法除去蛋白质

过氧化氢脱色;采用苯酚-硫酸显色

用分光光度法测定多糖含量。结果: 红花多糖的浓度与吸光度在5~50 μg·mL-1呈良好的线性关系

回归方程:Y=0.017 49X-0.031 65

r=0.999 4。多糖质量分数为96.048%。结论: 含量测定方法操作简便

灵敏度高

稳定

重现性好。该方法可为开展红花多糖的抗肿瘤作用研究提供理想的实验药物。

Abstract

Objective: To study the extraction and assaying of Safflower polysaccharide (SPS)

and to provide suitable experimental drug specimen for exploreing the anti-carcinoma effect of SPS. Method: Water extracting and ethanol precipitating methods were applied to extract polysaccharide from Safflower. By Sevage method

protein can be removed

and by oxidation method to decolor the polysaccharide. After phenol-sulphuric acid coloring

the polysaccharide content was determined by the method of absorption spectrometry. Result: The standard curve was liner in the concentration range of 5~50 μg·mL-1 with correlation coefficient of r=0.9994

the linear regression equation was Y=0.01749X-0.03165

the contents of polysaccharide from Safflower was 96.048%. Conclusion: This methods have advantages of simple operation

good stability

high accuracy and good reproducibility.This extraction technology could be used to extract Safflower polysaccharide for studies on its anti-carcinoma effect.

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Related Author

MIN Yun-jiang1

ZHOU Shou-biao2

ZHANG Yin-ping1

GAO Qiong1

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WEI Wei

JIN Ri-xian

CHEN Yan-jun

Related Institution

Key Laboratory of Modern Preparation of Traditional Chinese Medicine

School of Pharmacy,Nanchang University

School of Chemistry and Chemical Engineering, Liaocheng University

School of Traditional Chinese Medicine,Guangdong Pharmaceutical College

Department of Pharmacy,Hunan University of Traditional Chinese Medicine

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