Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line

YANG Chang-fu; LI Ya-dong; NIU Jian-zhao; WANG Xiao-bo; HUANG Chun-fang; TANG Bing-hua; ZHAO Pi-wen; Liu Xia; WANG Ji-feng

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Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line

更新时间：2024-01-04

- Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 8, Pages: 113-116(2010)
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- Published：2010
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YANG Chang-fu, LI Ya-dong, NIU Jian-zhao, et al. Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 113-116.
DOI：

YANG Chang-fu, LI Ya-dong, NIU Jian-zhao, et al. Influence of Zedoary Oil on Cell Cycle and Cathepsin K Expression in A549 Cell Line[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 113-116. DOI：

摘要

目的 :探讨莪术油对A549细胞增殖抑制作用及组织蛋白酶K表达的影响。方法 : MTT法观察莪术油对A549细胞增殖的抑制作用;流式细胞术检测细胞周期阻滞;Western blot检测组织蛋白酶K的表达情况。结果: 莪术油作用后①MTT法结果显示莪术油在60 mg ·L-1~200 mg ·L-1对A549细胞增殖有抑制作用

IC50随作用时间的延长显著前移。②流式细胞术结果出现凋亡峰

与未处理组细胞相比

给药组细胞G0/G1期细胞比例增加

S期细胞比例降低。③Western blot结果显示

莪术油处理细胞24 h后

组织蛋白酶K表达明显上调

100 mg ·L-1达最大值。结论 : 莪术油能抑制肺腺癌A549细胞增殖

阻滞细胞周期的作用

其机制可能与上调组织蛋白酶K表达水平有关。

Abstract

Objective: To explore the Zedoary oil on A549 cell line inhibiting the proliferation and cathepsin K expression. Method : The effects of Zedoary oil on the proliferation of lung adenocarcinoma cell line A549 were determined by MTT assay. The cells cyclic retardation were examined by using flowcytometry. Cathepsin K expression of level were detected by using western blot. Result : MTT showed that Zedoary oil had an inhibitory effect on the proliferation of A549 cells in 60~200 mg ·L-1. Flow cytometry showed that Zedoary oil increased the percentage of G0/G1 phase cells and reduced the percentage of S phase cells. The results of western blot showed that the expression of cathepsin K was up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls. Conclusion :Zedoary oil can significantly inhibit the growth of A549 cells

blocking the cell cycle. The effects may due to the up-regulation of cathepsin K.

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