Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC

ZHANG Jin-lian; HE Min; XIE Yi-hui; YAO Dong-qin; YU Run-min; ZHANG Di-feng

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Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC

更新时间：2024-09-23

- Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 68-70(2010)
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- Published：2010
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ZHANG Jin-lian, HE Min, XIE Yi-hui, et al. Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 68-70.
DOI：

ZHANG Jin-lian, HE Min, XIE Yi-hui, et al. Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 68-70. DOI：

摘要

目的 :建立高效液相法测定枳壳饮片中柚皮苷、橙皮苷和新橙皮苷定量分析方法。方法 :Ultimate 3000 Cl8色谱柱 (4.6 mm×250 mm

5 μm)

流动相乙腈-水(磷酸调pH 3)(23 ∶77)。检测波长283 nm

流速1.0 mL·min-1

柱温30 ℃。结果 :柚皮苷、橙皮苷和新橙皮苷的线性范围分别为0.316 4~1.582 4 μg (r=0.999 9)

0.041 2~0.206 0 μg (r=0.999 9)

0.188 0~0.940 0 μg(r=0.999 9)

平均回收率均大于96.11%。结论 :该方法专属性强

结果准确可靠

重现性好

可用于枳壳饮片中柚皮苷、橙皮苷和新橙皮苷的含量测定

以便更好的控制枳壳饮片的质量。

Abstract

Objective: To establish the method of determining the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces by HPLC. Method : Ultimate 3 000 Cl8 column (4.6 mm×250 mm

5 μm)

mobile phase was acetonitrile-water (pH was adjusted to 3 by phosphoric acid) (23 ∶77). Wavelength was 283 nm

flow rate was 1.0 mL·min-1

column temperature was 30 ℃. Result: naringin

hesperidin and neohesperidin linear ranges were 0.316 4~1.582 4 μg(r= 0.999 9)

0.041 2~0.206 0 μg (r =0.999 9)

0.188 0~0.940 0 μg (r= 0.999 9). The average coefficient of recovery were greater than 96.11%. Conclusion : This method is highly specific

accurate and reliable and has good repeatability.It is suitable for the determination of the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces.It can be widely used for the quality control of Fructus Auranti pieces.

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Related Author

ZHANG Jin-lian

HE Min

XIE Yi-hui

YAO Dong-qin

YU Run-min

ZHANG Di-feng

ZENG Qi

TAN Dao-peng

Related Institution

Jiangxi University of Traditional Chinese Medicine International Education College

National Institutes for Food and Drug Control

The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine

Jiangxi University of Traditional Chinese Medicine

Jiangxi University of Chinese Medicine，Research Center for Traditional Chinese Medicine Resources and Ethnic Minority Medicine

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