Comparison of 2 Polysaccharide Content Determining Methods in

LIU Qian; BAI Su-fen; LA Xiao-jin; WANG Hui-fang; LIANG Jing; LA Wan-ying

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Comparison of 2 Polysaccharide Content Determining Methods in

更新时间：2024-01-04

- Comparison of 2 Polysaccharide Content Determining Methods in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 8, Pages: 32-34(2010)
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- Published：2010
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LIU Qian, BAI Su-fen, LA Xiao-jin, et al. Comparison of 2 Polysaccharide Content Determining Methods in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 32-34.
DOI：

LIU Qian, BAI Su-fen, LA Xiao-jin, et al. Comparison of 2 Polysaccharide Content Determining Methods in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 32-34. DOI：

摘要

目的: 选择一种准确、稳定、简便的二精丸多糖含量测定的方法。方法: 对硫酸-蒽酮法和苯酚-硫酸法测定二精丸多糖的含量进行比较。结果: 硫酸-蒽酮法和苯酚-硫酸法分别在1.2~12 mg·L-1(r=0.999 3)和1.5~15 mg·L-1(r=0.999 1)范围内呈线性关系;精密度试验RSD分别为0.13%和0.31%;硫酸-蒽酮法在3 h(RSD=2.87%)内稳定

苯酚-硫酸法在2 h(RSD=2.89%)内稳定;含量测定结果分别为53.79%和58.92%。结论: 苯酚-硫酸法测定二精丸多糖含量显色灵敏

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操作简便

可作为测定二精丸多糖含量测定的方法。

Abstract

Objective: To select a accurate stable and convenient method for determining Polysaccharide content in Erjing Wan. Method: Polysaccharide content in Erjing Wan was determined by two methods: athranone-sulfuric acid method and phenol-suifuric acid method. Result: The sugarin athranone-sulfuric acid method and phenol-suifuric acid method was liner in the range of 1.2~12 mg·L-1(r=0.999 3) and 1.5~15 mg·L-1(r=0.999 1) respectively. Athranone-sulfuric acid method was stable in 3 h

phenol-suifuric acid method was stable in 2 h; the sugar content was 53.79% and 58.92% respectively. Conclusion: Phenol-suifuric acid method is sensitive、stable and convenient and can be applied to determine polysaccharide content in Erjing Wan.

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Related Institution

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