Objective: To investigate the relativity between the body surface characteristics indicated by Evans blue and the hemorrheological parameters in rats with blood-stasis syndrome induced by macromolecule dextran. Methods: The rats were treated by injected 10% macromolecule dextran solution confected by 0.5% Evans blue

10% macromolecule dextran solution confected by sodium chloride

0.5% evans blue

or sodium chloride respectively through the caudal vein

then observed and photoed the exosymptom change presented by Evans blue in ear and tongue of the rats

and detected the hemorrheologic variation of the rats at 30

60

120

and 240 minutes after the treatment

and analyzed the relativity between the body surface characteristics indicated by Evans blue and the hemorrheological parameters in rats with blood-stasis syndrome. Results: In the rats treated by 10% macromolecule dextran solution confected by 0.5% Evans blue

the blue petechia in rat auricle and tongue were observed after 30 minutes

which aggravated as the time went by

and the whole ear of rat changed to dark blue and the blue petechiae of tongue enlarged to more than half of tongue after 240 minutes

while in the rats treated by 0.5% Evans blue

the rat auricle was semitransparent and the tongue color was uniform. In therats treated by 10% macromolecule dextran solution confected by Sodium Chloride

the blood viscosity increased significantly in different shear rate in 30 and 60 minutes

and the plasma viscosity

the aggregation index of erythrocyte

and the integral of aggregation index significantly increased at 30

60

120

and 240 minutes compared with the rats injected by sodium chloride. The index of red blood cell deformation and the integral area of deformation index at 30

60

120

and 240 minutes in the rats injected by sodium chloride are slightly lower than that in rats treated by 10% macromolecule dextran solution confected by sodium chloride

though there was no significantly difference can be detected. Conclusion: The surface characteristics in rat model with blood-stasis syndromcan be observed directly through the exosymptom change of ear and tongue when 0.5% Evans blue was confected into the 10% macromolecule dextran solution

and it correlated with the degree of blood stasis showed by the hemorrheologic variation. It indicates that the exosymptom changes in rats with blood-stasis syndrome induced by macromolecule dextran can be reflected objectively by Evans blue.