Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry
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Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 1, Pages: 39-45(2010)
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Published:2010
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NI Shu-mao, QIAN Da-wei, SHANG Er-xin, et al. Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 39-45.
DOI:
NI Shu-mao, QIAN Da-wei, SHANG Er-xin, et al. Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 39-45.DOI:
Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry
Objective: To analyze and identify the chemical composition of Dachunxiong Fang by LC-MS. Method :The analysis was performed on an AQCUITY UPLC BEH C18(2.1 mm×100 mm
1.7 μm) column.The mobile phases were acetonitrile and water in gradient elution.ESI source was used and data were acquited in positive and negative mode. Result :The structures of 17 compounds of Dachunxiong Fang were identified by direct comparison with MS and MS2 of Q-TOF
the elemental compositions analysis
the standard substance and the data of the literature. Furthermore
the types of the compounds were phenols
organic acids
phthalides and compounds containg nitrogen. Addition
the mass spectrum characterization of the major chemical composition of Dachunxiong Fang are analyzed. Conclusion :It is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography
the accurate molecular weights measured by MS and other information
which can trace to the source of compounds in Dachuanxiong Fang.
Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry
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Related Author
TANG Yu-ping
GUO Jian-ming
NI Shu-mao
QIAN Da-wei~
SHANG Er-xin
DUAN Jin-ao~
Lan SHEN
Shi-yu MA
Related Institution
Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine
School of Pharmacy, Shanghai University of Traditional Chinese Medicine(TCM)
Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine
Engineering Research Center of Modern Preparation Technology of TCM under Ministry of Education, Shanghai University of TCM
Lab.of Modern Preparation of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Ministry of Education,Nanchang