ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide
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ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 2, Pages: 56-59(2010)
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Published:2010
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YE Jin-xia, WANG Lan, YANG Bin, et al. ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 56-59.
DOI:
YE Jin-xia, WANG Lan, YANG Bin, et al. ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 56-59.DOI:
ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide
Objective: To investigate the antioxidant effects of water extract of Carthamus tinctorius L. on injury of cardiomyocytes induced by hydrogen peroxide (H2O2) and to detect reactive oxygen species (ROS) for exploring the mechanisms. Methods: In cultured neonatal rat cardiomyocytes
the antioxidant effect of water extract of Carthamus tinctorius L. was investigated after H2O2 (1 mmol·L-1) induced damage. The spontaneous beating
the survival rate and release of LDH
MDA
SOD
NO
NOS
GSH-Px and XOD enzyme activity in supernatant were investigated
and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR). Results: Preconditioning by water extract of Carthamus tinctorius L. increased the spontaneous beating and the survival rate
and decreased the release of LDH
MDA and SOD with the rise of SOD
NO
NOS and GSH-Px enzyme activity
while in the cell suspension ROS signal decreased significantly. Conclusion: Water extract of Carthamus tinctorius L. has antioxidative effect. The mechanisms are likely related with scavenging of free radicals
ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide
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Mechanism of Trichosanthis Pericarpium Aqueous Extract in Protecting H9c2 Cardiomyocytes from Hypoxia/Reoxygenation Injury via PI3K/Akt/NO Signaling Pathway
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梁日欣
杨滨
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YE Jin-xia~
CAI Zhengyin
CHU Jun
CHU Quangen
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Related Institution
1,2),WANG Lan~
福建中医学院中西医结合研究院
Anhui University of Chinese Medicine
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine
School of Biomedical and Chemical Engineering, Liaoning Institute of Science and Technology