Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study

ZHANG Yan-yin; WU Jian-jun; WANG Peng-shan; XUE Na; SHU Chang; WU Hong-yan

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Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study

更新时间：2024-09-23

- Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 114-117(2010)
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- Published：2010
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ZHANG Yan-yin, WU Jian-jun, WANG Peng-shan, et al. Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 114-117.
DOI：

ZHANG Yan-yin, WU Jian-jun, WANG Peng-shan, et al. Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 114-117. DOI：

摘要

目的 :探索贞芪酪蛋白肽(ligustri-astragali casein co-peptid

LACP)复合物对人肺腺癌A549细胞分化的诱导作用及作用机制。方法 :MTT法检测贞芪酪蛋白肽含药血清5%

10%

15%

20%浓度对人肺腺癌A549细胞增殖的影响;通过贞芪酪蛋白肽药物血清对A549细胞核质比和端粒酶活性的影响观察其诱导分化作用;免疫细胞化学方法观察贞芪酪蛋白肽药物血清对A549细胞Bax/bcl-2基因表达的影响。结果 :4种不同浓度的贞芪酪蛋白肽含药血清均对A549细胞增殖有抑制作用

其中以浓度为20%的贞芪酪蛋白肽血清的抑制率最高;能降低细胞的核质比

并抑制人肺癌细胞端粒酶活性的作用;贞芪酪蛋白肽血清能促进A549细胞凋亡基因bax/bcl-2的表达

与无药血清对照组比较

差异有统计学意义(P<0.01)。结论 :贞芪酪蛋白肽含药血清对人肺腺癌细胞A549有一定抑制作用;有诱导人肺癌细胞A549分化的作用

其主要机理可能是抑制了A549细胞端粒酶活性;贞芪酪蛋白肽诱导A549细胞凋亡的作用机制之一可能是促进促凋亡基因bax/bcl-2的表达。

Abstract

Objective: To explore the effect of ligustri-astragali casein co-peptid(LACP) on human lung adenocarcinoma A549 cell differentiation and the related mechanism. Method : MTT assay for human lung adenocarcinoma A549 cells; containing 5%

10%

15%

20% concentration of LACP serum was used. The influence of LACP containing serum on differentiation was evaluated by investigation of cellular nuclear-mass ratio and telomerase activity. Immunocytochemical method was used to observe the effect of LACP drug serum on the A549 Bax/bcl-2 expression. Results : The four kinds of different concentrations of LACP serum inhibited A549 cell proliferation. Among them

20% of LACP showed the highest inhibitory rate; and reduced the cells nuclear -mass ratio. LACP serum promoted A549 cell bax/ bcl-2 gene expression for apoptosis

and compared with the serum-free control group

the difference was significant ( P <0.05). Conclusion : LACP containing serum has certain inhibitory effect on human lung adenocarcinoma A549 cells. LACP complex shows promotion to human lung cancer cell A549 differentiation. The mechanism may be linked to inhibition of telomerase activity. The complex induced apoptosis of A549 cells may be related to promotion of bax/bcl-2 gene expression.

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Related Author

ZHANG Yan-yin1

WU Jian-jun1

WANG Peng-shan2

XUE Na1

SHU Chang1

WU Hong-yan1

YAN Qixiang

ZHU Yeyan

Related Institution

Gansu College of Traditional Chinese Medicine

高台县宣化乡卫生院

Affiliated Hospital of Nanjing University of Chinese Medicine

The First Clinical Medical College，Nanjing University of Chinese Medicine

Jiangsu Province Hospital of Chinese Medicine

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