Objective: To develop a HPLC method for the determination of palmatine and berberine in Shulitong Granule. Methods: A Kromasil-C18(250 mm×4.6 mm

5 μm)column was used for the HPLC determination.The mobile phase consisted of acetonitrile-0.1 mol/L potassium dihydrogen phosphate (28 ∶72)

and the flow rate was 1 mL/min.The detection wavelength was 345 nm. Results: A good linear relationship was constructed in the range of 0.016~0.080 μg (r=0.999 8)

for Palmatine hydrochloride and in the range of 0.027~0.135 μg (r=0.999 9) for berberine hydrochloride. The average recovery of palmatine hydrochloride was 99.74% (RSD=2.67%

n=5)

and that of berberine hydrochloride was 103.53%

RSD=1.48%(n=5). Conclusion: The developed method is simple

reproducible

specific and suitable for the quality control of Shulitong Granule.