Determination of Content of Berberine Hydrochloride in the Mongolian Medicine Tufuling Qiwei Powder by RP-HPLC
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Determination of Content of Berberine Hydrochloride in the Mongolian Medicine Tufuling Qiwei Powder by RP-HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 9, Pages: 70-71(2010)
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Published:2010
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CHEN Hong-mei. Determination of Content of Berberine Hydrochloride in the Mongolian Medicine Tufuling Qiwei Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 70-71.
DOI:
CHEN Hong-mei. Determination of Content of Berberine Hydrochloride in the Mongolian Medicine Tufuling Qiwei Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 70-71.DOI:
Determination of Content of Berberine Hydrochloride in the Mongolian Medicine Tufuling Qiwei Powder by RP-HPLC
Objective: The RP-HPLC method for determination of Berberine HydrochLoride in mongoLian herbal drug Tufuling Qiwei Powder was established. Method: Xterra (3.9 mm×150 mm
4 μm) was used as chromatographic column
acetonitriLe-water (30 ∶70) as a mobile phase
The flow rate was 0.8 mL·min-1 and detection wavelength was at 345 nm. Result: The linear range of Berberine Hydrochloride was 19~228 mg·L-1
r=0.999 8.The average recovery rate was 101.8%
RSD 1.87% (n=5). Conclusion: The method was simple accurate and rapid with good reproducibility.
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