Objective :To establish HPLC-fingerprints and compare the differences of processed Rhizoma Atractylodis Macrocephalae. This study was aimed at supplying reference for the quality control of processing technology. Method :Kromasil C18(4.6 mm×250 mm

5 μm) column was used with gradient elution of acetonitrile - water as the mobile phase. The detection wavelength was 242 nm and flow rate was 1.0 mL ·min-1. The "Chromatographic Fingerprint Similarity Evaluation" software was used to analyze similarity and compare the fingerprints. Result :There are obvious identical peaks between fried and crude Rhizoma Atractylodis Macrocephalae. But the samples of different processed materials had obvious differences in fingerprints. Conclusion :The HPLC-fingrerprint is repeatable and feasible in analysis and can be used in quality control.