A serum pharmacological study on antioxidation effects of Huanglian Jiedu Tang

SONG Jue; LU Tong; XIE Lin; LIU Xiao-dong

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A serum pharmacological study on antioxidation effects of Huanglian Jiedu Tang

更新时间：2024-09-23

- A serum pharmacological study on antioxidation effects of Huanglian Jiedu Tang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 118-122(2010)
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- Published：2010
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SONG Jue, LU Tong, XIE Lin, et al. A serum pharmacological study on antioxidation effects of Huanglian Jiedu Tang[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 118-122.
DOI：

SONG Jue, LU Tong, XIE Lin, et al. A serum pharmacological study on antioxidation effects of Huanglian Jiedu Tang[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 118-122. DOI：

摘要

目的 :研究黄连解毒汤及其单味药对细胞氧化损伤保护作用的药效动力学

并对其血清药理学方法进行探讨。方法 :采用血清药理学方法

研究黄连、黄芩、黄连解毒汤含药血清对H2O2所致PC12细胞氧化应激损伤保护作用的时间-效应关系;并研究黄连、黄芩、黄连解毒汤提取物和小檗碱、黄芩苷纯品对氧化损伤保护作用的剂量-效应关系。结果 :各药的含药血清对细胞氧化损伤都有明显的保护作用

其中以黄连解毒汤作用最强

黄芩和黄芩苷次之

黄连最弱;在试验浓度范围内

黄连解毒汤和黄芩提取物对模型细胞有剂量依赖性保护作用

而黄连没有保护作用;在0.15~10 μg ·mL-1浓度范围内

黄芩苷对细胞模型有保护作用

且呈剂量依赖性。0.15~10 ng ·mL-1浓度范围内

小檗碱没有明显的保护作用。结论 :黄连解毒汤体内抗氧化作用强于各单味药;黄芩苷可能是黄芩及其含药血清中的抗氧化成份之一

而小檗碱与含药血清的抗氧化作用无关;黄连解毒汤及其单味药含药血清对细胞氧化应激模型的保护作用呈时间和剂量依赖性。

Abstract

Objective: To study protective effects of Huanglian Jiedu Tang (HLJDT) and its components Coptidis Rhiyoma(CR)

Scutellariae Redix (SR) on oxidative stress- induced PC12 cell injury. Methods : Serum pharmacological method was used to study effects of HLJDT

SR on the cell oxidative injury. The herb-contained serum

obtained at different time after dosing in rats

was added to the cultured oxidative PC12 cells. The viability of PC12 cells was determined by MTT colormetric microassay. Effects of decoctions of HLJDT

herberine and baicalin were investigated as well. Result: The herb-contained serum of HLJDT

baicalin and the decoctions of HLJDT

baicalin had protective effects on cell injury so did baicalin at 0.15~10 μg ·mL-1. But herberine and the decoctions of CR had no effect. Conclusion: Obtained serum after ig. HLJDT and its components in rats have protective effect on oxidative stress-induced PC12 cell injury in a dose-dependent and time-dependent manner. Baicalin may act as an antioxidant in the herb-contained serum of HLJDT as well as in the decoctions.

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Related Author

SONG Jue1

LU Tong2

XIE Lin3

LIU Xiao-dong3

Wei HUANG

Han LI

Ben-yue LI

Yang ZHANG

Related Institution

The College of Pharmacy of Anhui Medical University

DMPKCenter,Shanghai Institute of Materia Medica,Chinese Academy of Science

China Pharmaceutical University Key Laborartory of Drug Metabolism&Pharmacokinetic

Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine

School of Basic Medicine, Guangzhou University of Chinese Medicine

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