Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method

WEI Hui-zhen; LI Xin-nan; GONG Jian-ping; WANG Yue-sheng; ZHANG Hong-hong; RAO Yi; XIE Fei

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Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method

更新时间：2024-09-23

- Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 39-43(2010)
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- Published：2010
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WEI Hui-zhen, LI Xin-nan, GONG Jian-ping, et al. Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 39-43.
DOI：

WEI Hui-zhen, LI Xin-nan, GONG Jian-ping, et al. Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 39-43. DOI：

摘要

目的 :建立紫外分光光度法测定罗银胶囊中总黄酮含量的测量不确定度评定方法。方法: 通过紫外分光光度法测定罗银胶囊中总黄酮含量的测定及方法学验证数据

对来自对照品溶液配制、样品前处理、显色反应、标准曲线拟合及回收率因素所引入的不确定度进行评估

由此计算合成不确定度

最终给出测量结果在95%置信区间下的扩展不确定度。结果: 本次实验的罗银胶囊中总黄酮含量测定结果为(31.13±1.61) mg ·g-1。结论: 建立的不确定度评定法适用于紫外分光光度法测定中药含量的不确定度分析中。

Abstract

Objective: To evaluate the uncertainty of measurement for determination of total flavonoids in Luoyin soft capsules by UV spectrometery. Method: Through establishing UV spectrometery method determination mode

the factors affecting the uncetainty were deduced

including standard solution preparation

sample pretreatment

color reaction

calibration curve and recovery tests. Each component of uncertainty was evaluated

from which the combined uncertainty and finally the extended uncertainty were obtaine in 95% confidence intervals. Result : The determined content of total flavonoids in Luoyin soft capsules of this test is (31.13±1.61) mg ·g-1. Conclusion: The established mathematic model is applicable to the uncertainty analysis of UV spectrometery method in the determination of traditional Chinese medicine.

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Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method

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Related Author

LI Xin-nan2

GONG Jian-ping1

WANG Yue-sheng1

ZHANG Hong-hong1

RAO Yi1

XIE Fei2

WEI Hui-zhen1

LI Peng

Related Institution

The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine

Jiangxi Traditional Medicine

College of Pharmaceutics,Ningxia Medical University

Ningxia Research Center of Modern Hui Medicine Engineering and Technology

Ningxia Research Institute of Medicine & Pharmacy,Ningxia Medical University

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