Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 1, Pages: 18-21(2010)
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Published:2010
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LIU Wei, GUO Lei, FAN Ting, et al. Effect of Different Fertilizers on Content of in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 18-21.
DOI:
LIU Wei, GUO Lei, FAN Ting, et al. Effect of Different Fertilizers on Content of in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 18-21.DOI:
Objective: Determine the content of Saikosaponin a and d by HPLC-ELSD.To analyze different methods of fertilization of the programme as optimum Fertilization one of the targets. Method: The HPLC method was used
chromatography condition was:Agilent TC-C18(4.6 mm×250 mm
5 μm)column
acetonitrile-water (50∶50) as mobile phase
detection is ELSD
drift temperature 93 ℃
air flow 2.5 L·min-1
column temperature 20 ℃. Results :Establishing the fertilizer programme of Radix Bupleuri. Conclusion :The method is accuate
simple
and can provide the basis for optimization method of fertilization scheme by changes of Saikosaponin a and d.