Objective: To study the mechanism of the Chinese medicine Weichangshu on mitochondrial kinetic energy of stomach in diabetic mellitus (DM) rats by measuring the changes in morphology

structure

function and protein of mitochondria. Method: Standard Sprague Dawley rats (150-170 g) were randomly divided into 4 groups

including a control group

DM model group

DM+ WeichangshuⅠgroup (750 mg·kg-1)

DM+ Weichangshu Ⅱ group (1 500 mg·kg-1). The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes in mitochondrial transmembrane potential

respiratory chain and mitochondrial proteins were detected using flow cytometry

fluorescence labeling and Western blot respectively. Result: Weichangshu could ameliorate the mitochondrial morphology

structure and respiratory chain of stomach in diabetic mellitus rats. Mitochondrial transmembrane potential

ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 was up-regulated and the expression of bax was down-regulated. Conclusion: Weichangshu could promote the gastrointestinal motility

which might be related with the regulation of mitochondrial kinetic energy.