Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis

LI Xiao-lin; SHAO Ai-juan; CHEN Min; HUANG Lu-qi

您当前的位置：

首页 >

文章列表页 >

Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis

更新时间：2024-09-23

- Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 1, Pages: 14-17(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
LI Xiao-lin, SHAO Ai-juan, CHEN Min, et al. Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 14-17.
DOI：

LI Xiao-lin, SHAO Ai-juan, CHEN Min, et al. Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 14-17. DOI：

摘要

目的 :建立适用于酸浆种子的SDS-PAGE和2-D PAGE蛋白样品提取方法。方法: 比较直接提取法、TCA-丙酮法、Tris-丙酮法、饱和酚法、改良饱和酚法等7种提取方法所获酸浆种子总蛋白含量以及SDS-PAGE电泳的谱带数目、强度和电泳分辨率等方面的差异。结果: HEPES buffer直接提取法提取蛋白效率较高

杂质少

电泳谱带清晰

分辨率高;PVP buffer和Lysis buffer A直接提取法次之

但杂质较多;TCA-丙酮法、Tris-丙酮法、饱和酚法和改良的饱和酚法差别不大

蛋白纯度较高

但提取效率低

谱带数目少;此外

Lysis buffer A法、丙酮法和酚法易产生高丰度蛋白的干扰。结论: 直接提取法中的HEPES buffer法最好

优于丙酮法、酚法和其它直接提取法。

Abstract

Objective: To compare seven protein extraction protocols(PVP buffer

HEPES buffer

Lysis buffer A extraction and TCA/acetone

tris-base/acetone

phenol/ammonium acetate

acetone/phenol/ammonium acetate precipitation) and establish a suitable one for total proteins extraction from Semen Seu Fructus Physalis. Methods: The protein yield

protein purity and SDS-PAGE patterns was determined and analyzed. Results: HEPES buffer extraction produce the purer sample

much more clear bands and highest resolution. PVP buffer and Lysis buffer A extraction had most impurities. TCA/acetone

tris-base/acetone

phenol/ammonium acetate

acetone/phenol/ ammonium acetate precipitation methods had the purest sample

but protein yield and band numbers were lowest. Besides

the methods employing Lysis buffer was easy to produce high-abundance proteins and interfered in the electrophoresis resolution. Conclusion: HEPES buffer extraction was the best sample preparation protocol for SDS-PAGE and 2-D PAGE.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparison of Protein Extraction Methods for the Semen Seu Fructus Physalis

Related Author

LI Xiao-lin

SHAO Ai-juan

CHEN Min

HUANG Lu-qi~

Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰