Study on Dynamics Trends of Protocatechuic Acid Content of the  Rupr. in Different Growth Stages

Wang Qin; WEI Qing-hua

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Study on Dynamics Trends of Protocatechuic Acid Content of the Rupr. in Different Growth Stages

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- Study on Dynamics Trends of Protocatechuic Acid Content of the Rupr. in Different Growth Stages
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 54-56(2010)
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- Published：2010
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Wang Qin, WEI Qing-hua. Study on Dynamics Trends of Protocatechuic Acid Content of the Rupr. in Different Growth Stages[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 54-56.
DOI：

Wang Qin, WEI Qing-hua. Study on Dynamics Trends of Protocatechuic Acid Content of the Rupr. in Different Growth Stages[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 54-56. DOI：

摘要

目的 :为锁阳药材最佳采药期的确定提供科学依据。方法 :用高效液相色谱法

对不同地区、不同生育期锁阳的原儿茶酸含量的动态变化进行了研究。色谱条件Lichrosorb ODS C18 柱(4.6 mm×250 mm

5 μm);检测波长259 nm;流动相乙腈-水-冰醋酸(10 ∶100 ∶0.1);流速1.0 mL ·min-1;进样量10 μL。结果 :不同地区、不同生育期锁阳的原儿茶酸含量呈现规律性变化

出土前的原儿茶酸含量最高

出土期次之

结实期最低。结论 :可将原儿茶酸可以作为锁阳的指标性成分之一

如仅以原儿茶酸作为指标成分

则出土前采集的锁阳药材质量最好

该结果为锁阳药材采收期的确定和质量评价提供了科学依据。

Abstract

Objective: To provide scientific foundation for establishing harvesting time of best quality of herb. Method: Protocatechuic acid was determined by HPLC to study dynamic trends of its content of the Cynomorium songaricum in different growth stages. Using Eclipse XDB C18(4.6 mm×250 mm

5 μm)as the stationary phase

acetonitrile-water-glacial acetic acid(10 ∶100 ∶0.1)as the mobile phase at a flow rate of 1.0 ml ·min-1

259 nm as the detection wavelength

the injection volume was 10 μL. Result: The protocatechuic acid content of C. songaricum Rupr. changed regularly. The content was higher at preemergence stage than that of unearthed. The content at unearthed stage was higher than that in flowering stage. And In flowering stage was higher than fruiting stage. Conclusion: Protocatechuic acid could be regarded as one of mark components

that the preemergence stage was the best. The research provided a scientific foundation for the right harvesting -time and the quality evaluation of Herba cynomorii.

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Related Author

WANG Qin 1

WEI Qing-hua2

WANG Zhao-hong2

XING Jun-bo1

HUANG Xuan

HUANG Can-lin

PEI Xiang-ping

PEI Miao-rong

Related Institution

Zhangye Institute for Drug Control

Zhangye Medical College

Shaanxi Traditional Chinese Medicine

Shanxi College of Traditional Chinese Medicine

山西中医学院山西太原030024

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