Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca Concentration on rat Cardiomyocytes

LIANG Tao1; FANG Tai-hui1; YAO Xiu-juan2; XU Li1; YUAN Dong-ping1; QIU Rong-li1; ZHOU Ling-ling1

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Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca Concentration on rat Cardiomyocytes

更新时间：2024-01-04

- Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca Concentration on rat Cardiomyocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 12, Pages: 94-97(2009)
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LIANG Tao1, FANG Tai-hui1, YAO Xiu-juan2, et al. Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca Concentration on rat Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(12): 94-97.
DOI：

LIANG Tao1, FANG Tai-hui1, YAO Xiu-juan2, et al. Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca Concentration on rat Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(12): 94-97. DOI：

摘要

目的:研究环维黄杨星D(CVB-D)对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用和对急性分离大鼠心肌细胞内游离Ca2+浓度的影响。方法:采用培养的乳鼠心肌细胞建立缺氧/复氧损伤模型

测定心肌细胞搏动频率、细胞存活率、肌酸激酶(CK)的含量;应用特异性荧光指示剂Fluo-3/AM负载急性分离的大鼠心肌细胞

用激光共聚焦显微镜检测胞内游离钙的变化。结果:缺氧/复氧损伤+环维黄杨星D组(H/R+CVB-D)乳鼠心肌细胞搏动频率、细胞存活率与缺氧/复氧损伤组比较明显升高

CK含量与缺氧/复氧损伤组比较明显下降。对急性分离大鼠心肌细胞内[Ca2+]i逐步升高

并呈剂量依赖性;在有外钙和无外钙时

CVB-D对胞内钙的升高有显著差异(P<0.05);在无外钙并加入内罗啶孵育后

CVB-D引起的胞内[Ca2+]i轻度升高

但与无钙组相比无显著性差异(P>0.05)。结论:环维黄杨星D对培养乳鼠心肌细胞缺氧/复氧损伤有保护作用

对急性分离大鼠有升高心肌细胞内游离钙离子浓度的作用

[Ca2+]的升高既源于内钙释放又源于外钙内流。

Abstract

Objective:To study the protective effects of Cyclovirobuxine D exposed to hypoxia-reoxygenation injury on the cultured myocardial cells and effect of intracellular free Ca2+ concentration on rat acute isolated cardiomyocytes.Methods: The hypoxia/reoxygenation(H/R) injury model of cultured neonatal rat cardiomyocytes was develop.Myocardial cells beating rate

cell viability

CK were measured.Rat acute isolated cardiomyocytes was measure intracellular free Ca2+concentration by calcium fluorescent probe Fluo-3/AM and laser confocal microscope.Results: Compared with H/R group

beating rate

cell viability of H/R+CVB-D was significantly increased.Compared with H/R group

CK of H/R+CVB-D was significantly reduced.In Tyrode CVB-D can elevate [Ca2+]]i

its peak values which indicate that the level of [Ca2+]]i act in an dose-dependent.[Ca2+]iwas also signficantly higher in Tyrode than that in no calcium Tyrode(P<0.05).In no calcium Tyrode with ryanodine

CVB-D had no effect on level of [Ca2+]]i compared with group of no calcium Tyrode(P>0.05).Conclusion: CVB-D could protect cultured cardiomyocytes against H/R injury.CVB-D was found to increase rat acute isolated intracellular free Ca2+ concentration.The increasing of [Ca2+]]i was caused by releasing from intracellular stores and extracellular Ca2+ influx.

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