Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction

YI Jia-li; ZHANG Zhong-ai; LUO Tian-jiong; LIU Bao-lin

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Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction

更新时间：2024-09-23

- Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 202-206(2010)
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- Published：2010
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YI Jia-li, ZHANG Zhong-ai, LUO Tian-jiong, et al. Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 202-206.
DOI：

YI Jia-li, ZHANG Zhong-ai, LUO Tian-jiong, et al. Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 202-206. DOI：

摘要

目的 :观察丹桔胶囊对血管紧张素Ⅱ(AngⅡ)损伤血管内皮功能的保护作用。方法 :制备大鼠胸主动脉环

观察丹桔胶囊提取剂对抗AngⅡ引起的胸主动脉环收缩反应;AngⅡ刺激人脐静脉血管内皮细胞(HUVEC)以反转录—聚合酶链反应( RT-PCR) 方法检测HUVEC的内皮素(ET-1)mRNA 表达水平;蛋白质印迹(Western blot)方法检测HUVEC中细胞外信号调节激酶(extracellular signal-regulated kinase

ERK)和核转录因子κB (nuclear factor κB

NF-κB)的活化情况。结果 :AngⅡ引起胸主动脉收缩

丹桔胶囊制剂能显著对抗血管收缩作用

下调AngⅡ刺激所致的HUVEC细胞ET-1 mRNA 过度表达

抑制ERK1/2磷酸化

同时对NF-的活化表现出显著的抑制作用。p65位移、IκB-α磷酸化降解

丹桔胶囊上述抑制作用均呈现浓度依赖性。结论 :丹桔胶囊抑制AngⅡ对血管内皮功能的损伤

其机制与降低ET-1表达

抑制ERK/NF-κB途径活化有关。

Abstract

Objective: To observe the protective effect of Danju Capsule on vascular endothelial dysfunction induced by angiotensinⅡ(AngⅡ). Method: The Ang Ⅱ-induced contraction of rat aortic rings was tested in tissue bath. The expression of endothelin ( ET)-1 mRNA was evaluated by RT-PCR in human umbilical vein endothelial cells (HUVEC) stimulated by Ang II. The activation of extracellular signal-regulated kinase (ERK) and nuclear transcription factor (NF-κB) and its inhibitor (IκB-α) were detected by Western blot in HUVEC. Result: The results showed that 1×10- 7 mol ·L-1 Ang Ⅱ increased contraction of rat aortic rings

ET-1mRNA expression

ERK1/2 phosphorylation

NF-κB p65 displacement and IκB-α phosphorylation degradation

which were significantly inhibited by Danju Capsule in a dose-dependent manner. Conclusion: Ang Ⅱ may be involved in the initiation and progression of cardiovascular diseases by injuring vascular endothelium directly and causing endothelial dysfunction. Danju Capsule was shown to protect against Ang Ⅱ induced endothelial dysfunction

suggesting that Danju Capsule may play a role in the prevention and treatment of cardiovascular diseases.

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Related Author

YI Jia-li1

ZHANG Zhong-ai2

LUO Tian-jiong2

LIU Bao-lin1

ZHANG Yuanyuan

JIN Peipei

BIAN Dong

GUO Dengzhou

Related Institution

Department of Gerontology,Hospital of Traditional Chinese Medicine of Nanjing

Department of Pharmacology of Chinese Materia Medica,China Pharmaceutical University

The First Affiliated Hospital of Hebei University of Chinese Medicine/Hebei Provincial Hospital of Traditional Chinese Medicine

Graduate School， Hebei University of Chinese Medicine

Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine

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