Determination the Five Kinds of Rhubarb Anthraquinonesin Yankening Tablets by HPLC

YIN Yong-qin; YAN You-shao; SHEN Zhi-bin; CUI Hong-hua; FANG Cui-ting

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Determination the Five Kinds of Rhubarb Anthraquinonesin Yankening Tablets by HPLC

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- Determination the Five Kinds of Rhubarb Anthraquinonesin Yankening Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 122-124(2010)
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- Published：2010
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YIN Yong-qin, YAN You-shao, SHEN Zhi-bin, et al. Determination the Five Kinds of Rhubarb Anthraquinonesin Yankening Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 122-124.
DOI：

YIN Yong-qin, YAN You-shao, SHEN Zhi-bin, et al. Determination the Five Kinds of Rhubarb Anthraquinonesin Yankening Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 122-124. DOI：

摘要

目的: 建立同时测定炎可宁片中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量的高效液相色谱法。方法: 色谱柱Kromasil C18(4.6 mm×250 mm

5 μm);流动相甲醇(A)-0.1%的磷酸水(B)溶液

采用梯度洗脱

0~10 min

75%~85% A

10~20 min

85%~90% A;流速1.0 mL ·min-1

柱温25 ℃

检测波长254 nm。结果: 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.016 0~0.096 0 μg

0.012 0~0.072 0 μg

0.016 0~0.096 0 μg

0.036 4~0.218 4 μg

0.015 6~0.093 6 μg与峰面积呈良好的线性关系;平均回收率分别为96.6%(RSD 0.76%

n=5)、97.2%(RSD 1.35%

n=5)、96.9%(RSD 1.57%

n=5)、98.1%(RSD 1.25%

n=5)和96.2%(RSD 0.62%

n=5)。结论: 本方法快速简便

专属性强

可作为炎可宁片质量控制方法之一。

Abstract

Objective: To determine the content of aloe-emodin

rhein

emodin

chrysophanol and physcion in Yankening Tablets by HPLC. Method: Using Kromasil C18 column (4.6 mm×250 mm

5 μm)

the methanol(A)-0.1%phosphoric acid (B)was used as the mobile phase. Using gradient elution

0~10 min

75%~85% A

10~20 min

85%~90% A. The flow rate was 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was 254 nm. Result: The linearity of this method were for 0.016-0.0960 mg

0.120-0.072 0 μg for rhein

0.016 0-0.096 0 μg for emodin

0.036 4-0.218 4 μg for chrysophanol

0.015 6-0.093 6 μg for physcion. There coveries of aloe-emodin

rhein

emodin

chrysophanol and physcion were 96.6%

97.2%

96.9%

98.1% and 96.2%. RSD were 0.76% (n=5)

1.35% (n=5)

1.57% (n=5)

1.25% (n=5) and 0.62% (n=5)

respectively. Conclusion: This method was rapid

accurate and can be used as one kind of Yankening Tablets’ quality control method.

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Related Author

YIN Yong-qin

YAN You-shao

SHEN Zhi-bin

CUI Hong-hua

FANG Cui-ting

ZHANG Da-jun

WANG Zhao-hua

LI Xiang-yu

Related Institution

College of Chinese Materia Medica,Guangdong Pharmaceutical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmacy， Jiangxi University of Chinese Medicine

Jiangxi Jingde Traditional Chinese Medicine Co. Ltd.

Jilin Agricultural University

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