Objective:To study the mechanism of anti-hepatitis B virus by flavonoids extract from milfiol herb

we observed the rate of NF-κB positive cells and activity of GSH-PX on HepG2.2.15 cells in vitro.Method:The rate of NF-κB positive cells and activity of GSH-PX were determined by SABC immunohistochemistry and colorimetry respectively.Result: The rate of NF-κB positive cells increased significantly by treatment of flavonoids extract from milfoil herb at both high and low doses

the activity of GSH-PX increased significantly by treatment of flavonoids extract from milfoil herb at high dose while it increased to some extent for the low dose treatment.Conclusion: The anti-hepatitis B virus effect of flavonoids extract from milfoil herb may be related to increasing of the rate of NF-κB positive cells and the activity of GSH-PX.HepG2.2.15 cells.