Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats
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Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 5, (2010)
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Published:2010
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gongmingyu. Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5).
DOI:
gongmingyu. Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5).DOI:
Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats
ABSTRACT: Objective: To explore protective effect and mechanism of breviscapine against myocardial ischemia reperfusion injury in rats. Methods: 40 SD rats were divided into sham operation group
ischemia reperfusion group and breviscapine group randomly.Rats in breviscapine group were injected with breviscapine 25、50mg.kg1.d-1 respectively prior to ischemia reperfusion .other two groups were given NS instead of breviscapine equally at a week before the operation. Myocardial ischemia reperfusion injury model was established. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with AnnexinⅤ-EGFPand propidium iodiode ( PI) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Results : breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia reperfusion injury obviously(P<0.05,P<0.01);The degree of injuring on ultrastructures of myocardial cell in breviscapine group is rather alleviated than in ischemia reperfusion group.Conclusion : breviscapine may protect myocardial cells during ischemia reperfusion injury and the protective mechanism may be obtained by inhibiting myocardial apoptosis.
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Related Institution
Chengde Medical College
Department of Radiotherapy,Affiliated Hospital of Chengde Medical College
The First Hospital of Hunan University of Chinese Medicine