Objective:To establish a method for the determination of quercitrin and menthol in Shuangbaisan

and provide a reference for quality control and the chemical material foundation of Shuangbai preparations.Methods:The content of the quercitrin was determined by HPLC.Eclipse XDB C18 was used

with acetonitrile-0.01 mol·L-1 potassium dihydrogen phosphate-acetic acid(16∶84∶2.1)as a mobile phase

detection wavelength at 352nm

flow rate at 1mL·min-1

and column temperature at 40 ℃.With Borneolum syntheticum as the internal standard

GC with HP-5 column was used

sampling temperature at 210℃

FID detector temperature at 280℃

temperature programming started at 50 ℃

held for 2 min

increased at a rate of 60 ℃·min-1 to 120 ℃

held for 5 minites

increasd at a rate of 10 ℃·min-1 to 150 ℃

then increased at a rate of 60 ℃·min-1 to 235 ℃ and kept it for 10 minites

low rate at 1.1 mL·min-1

spilt ratio was 1∶1.Results: Good linearity for Quercitrin and Menthol were in the rang of 0.020 48～1.228 8 μg(r>=0.999 9) and 0.002 73～0.087 36 μg(r>=0.999 5)

respectively.The average recoveries were 97.20%(RSD=1.75 %) for quercitrin and 99.97%(RSD=2.88%[0]) for menthol.The concents of quercitrin and menthol in Shuangbaisan were 0.851 9～1.286 3 mg·g-1、0.068 3～0.855 6 mg·g-1

respectively. Conclusion:This method is reliable and simple

which provides a reference for the quality control of Shuangbaisan.