Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC

WANG Hong-zhi; FAN Jun-ting; LIU Yong; LI Wan-qing

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Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC

更新时间：2024-01-04

- Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 6, Pages: 24-25(2009)
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- Published：2009
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WANG Hong-zhi, FAN Jun-ting, LIU Yong, et al. Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(6): 24-25.
DOI：

WANG Hong-zhi, FAN Jun-ting, LIU Yong, et al. Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(6): 24-25. DOI：

摘要

目的:建立高效液相色谱法(HPLC)同时测定抗溶血颗粒中芍药苷与橙皮苷的含量。方法:色谱柱为TIANHE-C18(4.6mm×250mm

10μm)

流动相:甲醇-1%磷酸(35∶65)

检测波长270nm

流速为1.0mL·min-1

柱温为30℃。结果:芍药苷在0.1984～0.992μg范围内线性良好

r=0.9979;橙皮苷在0.2112～1.056μg范围内线性良好

r=0.9993;样品平均回收率为:橙皮苷99.08%

RSD=1.47%;芍药苷100.67%

RSD=1.60%。结论:高效液相法可靠、准确

专属性强

重复性好

可用于该制剂质量控制。

Abstract

Objective:To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods:A Tianhe C18 Column was used with methanol-1% Phosphoric acid (35∶65) as the mobile phase at the flow rate of 1.0ml/min.The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0.198 4 ～ 0.992 μg (r=0.997 9 )

The linearity of hesperidin was good in the range of 0.211 2 ～1.056 μg(r=0.999 3 ).The average recovery of paeoniflorin was 99.08%

RSD=1.47%(n=6).The average recovery of hesperidin was 100.67%

RSD=1.60%(n=6). Conclusion: The method is simple

reliable

accurate

and can be applied as the quality control method of Antihernolysis Granule.

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