Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin
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Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 15, Issue 5, Pages: 21-24(2009)
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Published:2009
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PAN Jun1, YU Qing-hua1, GUI Shuang-ying 1, et al. Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(5): 21-24.
DOI:
PAN Jun1, YU Qing-hua1, GUI Shuang-ying 1, et al. Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(5): 21-24.DOI:
Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin
Objective:To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin. Methods:HPLC method was used to analyze the content of total glycosides
the adsorption and desorption performance of D101
D201
D301
D401
AB-8
NKA-9 were investigated
and the purification of conditions were inspected. Results:The best macroporous resin for purified extractive of total glycosides in tree peony bark was D101
the concentration of Paeoniflorin was 1.83 mg·mL-1 with pH 6.5
and with the elution flow rate of 1 BV·h-1. Purification of the conditions were as follows:10 BV water and 4 BV 70% ethanol were used as eluant of paeoniflorin
respectively. Portions of 70% ethanol were collected
concentrated
and dried. Conclusion:D101 resin can be used to refine the total glycosides in the water extracts of tree peony bark.