Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules

WU Wei

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Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules

更新时间：2024-01-04

- Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 8, Pages: 24-26(2009)
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- Published：2009
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WU Wei. Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 24-26.
DOI：

WU Wei. Study on Qualitative and Quantitative Methods for Huazhuqingshen Granules[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 24-26. DOI：

摘要

目的:研究化浊轻身颗粒中主要药物的鉴别和含量测定方法。方法:采用薄层层析鉴别主要药物

用高效液相色谱法测定制剂中四羟基二苯乙烯苷的含量。结果:建立了制剂中黄芪、大黄、何首乌的鉴别方法

四羟基二苯乙烯苷的线性范围为0.1648～3.2960μg(r=0.9996);加样回收率为99.0%

(RSD为3.0%

n=6)。结论:该实验建立的鉴别和含量测定方法专属性强、重复性好

可用于该制剂的质量控制。

Abstract

Objective:To study the gualitative and quantitative methods of identification and assay Huazhuqingsheng Granules.Methods: The major compounds in this preparation were identified by the thin layer chromatographic methods and the content of 2

4′-tetrahydroxystilbene-2-O-β-D-glucoside was determined by high performance liquid chromatography.Results: Rhubarb、Fleecetlower root and milkvetch root were identified.The linear range of 2

4′-tetrahydroxystilbene-2-Ο-β-D-glucoside was within(0.164 8～3.2960 μg)(r=0.999 6.)The average recovey(n=6) was 99.0%(RSD=3.0%).Conclusion: The identification and determination methods established were specific

and repeatable.It could be used to control the quality of this preparation.

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Related Author

YAN Xue-sheng1

LI Yan

MA Zhong-yan2

HUANG Rui-song1

CHEN Yan-jun2

SU Qing1

DENG Ru-ming1

YANG Dong-ai1

Related Institution

Guangzhou University of Chinese Medicine

College of Pharmacy,Shenyang Pharmaceutical University

山东省中医药研究院

Shandong University of Traditional Chinese Medicine

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