Determination of Total Iridoid Glycoside in Ajuga decumbens by Spectrophotometry

WANG Jin-yu; SUN Xiao-li; TONG Yan; CHEN Liang-mian; ZHANG Kai-bing; WANG Zhi-min; LI Jiang-rong

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Determination of Total Iridoid Glycoside in Ajuga decumbens by Spectrophotometry

更新时间：2024-01-04

- Determination of Total Iridoid Glycoside in Ajuga decumbens by Spectrophotometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 8, Pages: 3-5(2009)
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- Published：2009
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WANG Jin-yu, SUN Xiao-li, TONG Yan, et al. Determination of Total Iridoid Glycoside in Ajuga decumbens by Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 3-5.
DOI：

WANG Jin-yu, SUN Xiao-li, TONG Yan, et al. Determination of Total Iridoid Glycoside in Ajuga decumbens by Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(8): 3-5. DOI：

摘要

目的:建立筋骨草药材中总环烯醚萜苷的含量测定方法。方法:采用分光光度法

以乙酰哈巴苷(8-acetylharpagide)为对照品

经酸水解、二硝基苯肼乙醇试液-氢氧化钠甲醇水溶液显色后

在586nm处测定其含量。结果:乙酰哈巴苷及筋骨草药材显色稳定

乙酰哈巴苷在0.0162～0.3248mg范围内线性关系良好(r=0.9998)

筋骨草药材样品平均回收率为101.2%

RSD=2.0%。结论:建立了比色法测定筋骨草药材中总环烯醚萜苷类含量的方法

该方法简便、准确、重复性好

可作为该药材的质量控制方法。

Abstract

Objective:To set up a method for the determination of the total iridoid glycoside in herb medicine of Ajuga decumbens Thunb.Methods: Spectrophotometric method was developed to determine the total iridoid glycoside.8-acetylharpagide was used as the reference substance

the samples were hydrolyzed by acid

4-dinitrophenylhydrazine ethanol test solution was used as color-developing agent

methanol-water solution of sodium hydroxide as solvent.The maximal absorption wavelength was 586 nm.Results: The absorbance color of 8acetylharpagide and herb sample solutions was stable after the development

there was a good linear relationship in the scope of 0.016 2～0.324 8 mg(r=0.999 8).The average recovery of the added reference substance was 101.2% and RSD was 2.0%.Conclusions: The developed method is simple

accurate

reproducible and effective for the quality control of Ajuga decumbens Thunb.

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