Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats
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Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 15, Issue 12, Pages: 57-61(2009)
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Published:2009
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LIU Su-xiang, REN Yan, ZHUANG Gui-bao, et al. Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(12): 57-61.
DOI:
LIU Su-xiang, REN Yan, ZHUANG Gui-bao, et al. Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(12): 57-61.DOI:
Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats
Objective:To investigate the effects of Shugan-liangxue prescription(SGLX) from traditional Chinese medicine combined with anastrozole(ANA) on the serum estradiol(E2) level and bone metabolism in ovariectomized(OVX) Sprague-Dawley(SD) rats.Methods:The postmenopausal animal model was established after OVX and testified by vaginal smear method.Six-month SD rats were randomly divided into model
SGLX
ANA
ANA+SGLX
alendronate sodium(AL) and sham groups.After 16 weeks intragastric administration
the bone mineral density(BMD) of lumbar vertebra and femur were measured by dual energy X-ray absorptiometry.The serum E2
bone gla protein(BGP) and prostaglandin E2(PGE2) were detected by radioimmunoassay and bone alkaline phosphatase(BAP)
carboxyterminal propeptide of type Ⅰprocollagen(PICP)
pyridinoline(PYD)
tartrate-resistant acid phosphatase(TRAP)and cross-linked carboxyterminal telopeptide of typeⅠcollagen(ICTP) were determined by ELISA.Results: Serum E2 level decreased in SGLX
ANA and ANA+ SGLX groups compared with the model group(P<0.05
P<0.01
P<0.001)
BMD had no significant difference compared with the model.In ANA+SGLX group
serum BGP level increased
PYD and PGE2 declined significantly compared with ANA group(P<0.05
P<0.05
P<0.01).PYD decreased greatly in SGLX and AL groups compared with the model(P<0.001
P<0.001).Conclusions: This study showed that SGLX combined with ANA decreased the serum E2 level
meanwhile had the effect to relieve the side reaction of bone induced by ANA.