Determination of Verticine and Verticinone in Wanbei Zhike capsule by HPLC-ELSD
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Determination of Verticine and Verticinone in Wanbei Zhike capsule by HPLC-ELSD
Chinese Journal of Experimental Traditional Medical FormulaeVol. 15, Issue 5, Pages: 3-5(2009)
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Published:2009
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ZHANG Jie, LI Cheng-wang. Determination of Verticine and Verticinone in Wanbei Zhike capsule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(5): 3-5.
DOI:
ZHANG Jie, LI Cheng-wang. Determination of Verticine and Verticinone in Wanbei Zhike capsule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(5): 3-5.DOI:
Determination of Verticine and Verticinone in Wanbei Zhike capsule by HPLC-ELSD
Objective:To develop an HPLC-ELSD method for the determination of verticine and verticinone in Wanbei Zhike capsule. Methods:The analysis was carried out on a Dikma C18 analytical column with the mobile phase consisting of acetonitrile-0.1% diethylamine (70∶30). The detector drift tube temperature was set at 93 ℃ and the nitrogen flow rate was 2.2 mL·min-1.Results:The recoveries for verticine and verticinone were 97.35% and 97.46% with RSD 1.24%and1.27%(n=5). Conclusion:The developed method is simple
rapid
replicable
and can be used for the quality control of the preparation.