Determination of Flavonoids in Fructus Aurantii Immaturus by RRLC

WANG Chun; LV Shu-yi; SONG Zhi-qian; WANG Chao; LIU Zhen-li

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Determination of Flavonoids in Fructus Aurantii Immaturus by RRLC

更新时间：2024-01-04

- Determination of Flavonoids in Fructus Aurantii Immaturus by RRLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 6, Pages: 1-3(2009)
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- Published：2009
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WANG Chun, LV Shu-yi, SONG Zhi-qian, et al. Determination of Flavonoids in Fructus Aurantii Immaturus by RRLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(6): 1-3.
DOI：

WANG Chun, LV Shu-yi, SONG Zhi-qian, et al. Determination of Flavonoids in Fructus Aurantii Immaturus by RRLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(6): 1-3. DOI：

摘要

目的:建立高分离度快速液相色谱法(RRLC)测定枳实中主要黄酮类成分含量的方法。方法:RRLC色谱条件为ZobaxExtend-C18(4.6mm×50mm

1.8μm)色谱柱

流动相为甲醇-2%乙酸(33∶77);检测波长283nm;流速1mL·min-1;柱温30℃。结果:27个批次样品中有19个批次中的主要黄酮类成分为橙皮苷、柚皮苷、新橙皮苷和柚皮芸香苷

按各成分含量比例它们又可以分为两组

一组以柚皮苷和新橙皮苷含量较高

另一组则以柚皮芸香苷和橙皮苷含量较高;其余8个批次的主要黄酮类成分为橙皮苷和柚皮芸香苷

不含有柚皮苷和新橙皮苷

并都以橙皮苷含量较高。各样品主要黄酮成分含量之和为12.4%～38.07%。结论:RRLC方法稳定可靠

分析时间是HPLC的三分之一。枳实中黄酮类成分的种类和各成分的含量比例是不同的。

Abstract

Objective:To develop a RRLC method for the content determination of flavonoids in Fructus Aurantii Immaturus. Method: RRLC was applied with a Zobax Extend-C18 colunmn by methanol-2% ethanoic acid (33∶77) as the mobile phase.The flow rate was 1.0 mL·min-1 .The column temperature was 30℃ and the UV detection wavelength was 283 nm. Result: In the 27 batches samples

19 batches contained hesperidin

narirutin

neohesperidin and naringin

and they can divide into two groups by the ratio of flavonoids: in one group the content of hesperidin and narirutin were higher

and in another group the content of neohesperidin and naringin were higher; The other 8 batches only contain hesperidin and narirutin

and the content of hesperidin was higher. The content range of 4 kinds of main flavonoids in each sample were from 12.4% to 38.07%. Conclusion: The method is stable and reliable

and the analysis time of RRLC method was one-third of that of the HPLC method. The types and the content ratios of flavonoids in Fructus Aurantii Immaturus were different.

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