Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design

CUI Han-ming; XIA Pi-fang; CHENG Hui-ping

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Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design

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- Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 10, Pages: 23-25(2008)
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- Published：2008
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CUI Han-ming, XIA Pi-fang, CHENG Hui-ping. Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 23-25.
DOI：

CUI Han-ming, XIA Pi-fang, CHENG Hui-ping. Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 23-25. DOI：

摘要

目的:优选丹蒲颗粒中丹参和赤芍的醇提取方法及工艺条件。方法:采用正交试验设计考察不同影响因素和水平对芍药苷和丹参酮ⅡA提取率的影响。结果:乙醇回流法芍药苷和丹参酮ⅡA的提取率高于渗漉法和超声法

正交试验筛选出最佳工艺为:8倍量的80%乙醇

回流提取1.5h

共提取3次。经3批试验验证

对芍药苷和丹参酮ⅡA的提取率分别达到91.2%±4.6%

89.6%±5.2%。结论:优选的醇提工艺简单、高效

对芍药苷和丹参酮ⅡA的提取率高。

Abstract

Objective:To optimize the extract technology of Danshen and Chishao in DanPu Keli with orthogonal design.Method:The different factors for the extracting rate of paeoniflorin and tanshinone ⅡA were researched with orthogonal design.Result:The refluence method is best one in all methods.The major influencing factors were the followings alcohol quantity

alcohol concentration

extraction time and extraction number.The best extracting technology was that adding eight times alcohol(80%)

to reflux 1.5 hour and totally to extract 3 times.Three batchs tests showed that the extracting rate of paeoniflorin and tanshinone ⅡA were 91.2%±4.6% and 89.6%±5.2%

respectively.Conclusion:The optimum extracting condition was simple with a high efficiency.

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Related Author

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Related Institution

Henan University of Chinese Medicine

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School of Clinical Medicine，Changchun University of Chinese Medicine

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