Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC
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Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 14, Issue 10, Pages: 4-5(2008)
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Published:2008
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SUN Yan-rong1, 2, WANG Yue-sheng2, et al. Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 4-5.
DOI:
SUN Yan-rong1, 2, WANG Yue-sheng2, et al. Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 4-5.DOI:
Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC
Objective:A HPLC method for determination of andrographolide's content in andrographolide-resin complexes was studied.Method:Hypersil C18 column was used with methanol-water(55∶45) as the mobile and the detection wavelength was set at 225 nm.Results:linear in a range of 0.0956~0.956 μg the regression equation for andrographolide was Y=230.4X+47.264 with r=0.999 4.The average recovery was 99.99% and the RSD was 0.88%.Conclusion:The method is reliable and reproducible.It may be used for the determ ination of andrographolide in resin complexes of andrographolide ion-exchange resin.