Determination of Luteolin and Rupestonic Acid in  by High Performance Liquid Chromatography

ZHANG Jie1; GU Zheng-yi2; HE Cheng-hui2

您当前的位置：

首页 >

文章列表页 >

Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography

更新时间：2024-01-04

- Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 4, Pages: 1-3(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2009
- 稿件说明：
移动端阅览
ZHANG Jie1, GU Zheng-yi2, HE Cheng-hui2. Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 1-3.
DOI：

ZHANG Jie1, GU Zheng-yi2, HE Cheng-hui2. Determination of Luteolin and Rupestonic Acid in by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 1-3. DOI：

摘要

目的:建立新疆一枝蒿中的主要有效成分木犀草素与一枝蒿酮酸的高效液相含量测定方法。方法:采用高效液相色谱法测定

Shim packODSC18(4.6mm×250mm

5μm);甲醇-0.4%磷酸(45∶55)

检测波长350nm

该条件下测定木犀草素;检测波长241nm

该条件下测定一枝蒿酮酸。结果:木犀草素在12.80～51.20μg·mL-1范围内

峰面积与进样浓度具有良好线性关系

r=0.9998

平均回收率为99.92%(n=9);一枝蒿酮酸在9.72～77.76μg·mL-1范围内线形关系也良好

r=0.9993

平均回收率为99.82%(n=9)。结论:本法专属性强

操作简便

结果准确。适用于检测新疆一枝蒿中木犀草素和一枝蒿酮酸的含量。

Abstract

Objective:To develop an HPLC method for detecting luteolin and rupestonic acid

two key active constituents of Artemisia rupestis L.Method:A Shimpack ODSC18 column(4.6 mm×250 mm

5 μm) was used.The mobile phase was methanol:0.4% phosphoric acid(45∶55).The detection wavelengths were at 350 nm for luteolin

and 241 nm for rupestonic acid

respectively.Result: For luteolin

the linearity was obtained over the range of 12.80～51.20 μg·mL-1 with r=(0.999 8).The average recovery was 99.92%(n=9);for rupestonic acid

the linearity was obtained over the range of 9.72～77.76 μg·mL-1 with r=(0.999 3).The average recovery was 99.82%(n=9).Conclusion: The method was proved to be simple

rapid

and reliable to determine the contents of luteolin and rupestonic acid in Artemisa rupestis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Acteoside and Luteolin in by HPLC

Determination of the Content of Luteolin and Diosmetin in by HPLC

Comparison of Differential Saponin Contents and Pro-angiogenic Activities on Zebrafish Between Freeze-dried and Sun-dried Panacis Quinquefolii Radix Decoction Pieces

Exploration on Determination and Quantity Transfer of Index Components in Benchmark Samples of Erdongtang

Determination and Risk Assessment of Six Hepatotoxic Pyrrolizidine Alkaloids in Verbenae Herba

Related Author

CAI Jin-ping

DONG Lin

SHENG Lin

LIU Ming-sheng

YANG Yan

HU Bo

YE Xiao-ling

LIU Ji-cheng

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng

Shanxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰