Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats
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Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 15, Issue 1, Pages: 32-36(2009)
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Published:2009
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ZHAO Zong-jiang, DOU Xiao-ni, ZHANG Xin-xue, et al. Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(1): 32-36.
DOI:
ZHAO Zong-jiang, DOU Xiao-ni, ZHANG Xin-xue, et al. Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(1): 32-36.DOI:
Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats
Objective:To observe the effects of Tangshenping Capsule on the expressions of MCP-1 and mRNA in streptozotocin induced diabetic nephropathy rats.Methods: 50 SD rats were randomly divided into normal control group(n=10)
model group(n=40).Then the saccessfully modeled rats were re-divided into model group
irbesartan group and Tangshenping Capsule group.After administration of 12 weeks
the rats were sacrificed
Pathological changes in renal tissue were observed by HE and Mallory Staining.Using immunohistochemistry and hybridization analysis
renal tissue MCP-1 protein and mRNA expression were determined.Results: The model group showed reduction in appetite
activities
urine volume
body weight and pathological damages in kidney.Irbesartan and Tangshenping Capsule improved the above mentioned changes.Tangshenping Capsule group was superior to irbesartan group.There are some differences;in normal group and the treatment group renal tubular epithelial cells and interstitial cells showed mild MCP-1 and mRNA expression
in the model group there was a strong expression.Tangshenping Capsule significantly reduced the MCP-1 and mRNA expression(P<0.05).Conclusion: Tangshenping Capsule plays a role in treatment and prevention of DN via regulating MCP-1 and mRNA balance.
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Related Institution
Beijing Aerospace General Hospital
Beijing University of Chinese Medicine
School of Traditional Chinese Medicine,Southern Medical University
Zhujiang Hospital of Southern Medical University
Beijing Hospital of Traditional Chinese Medicine(TCM) Affiliated to Capital Medical University