Study on Determination of Polysaccharide in (P.Karst.) Fiasson

ZHU Yin-long; CHEN Ping; ZHANG Ya-huan

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Study on Determination of Polysaccharide in (P.Karst.) Fiasson

更新时间：2024-01-04

- Study on Determination of Polysaccharide in (P.Karst.) Fiasson
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 9, Pages: 1-3(2008)
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- Published：2008
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ZHU Yin-long, CHEN Ping, ZHANG Ya-huan. Study on Determination of Polysaccharide in (P.Karst.) Fiasson[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 1-3.
DOI：

ZHU Yin-long, CHEN Ping, ZHANG Ya-huan. Study on Determination of Polysaccharide in (P.Karst.) Fiasson[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 1-3. DOI：

摘要

目的:用紫外分光光度法建立稀针嗜蓝孢孔菌多糖的定量分析方法。方法:采用苯酚-硫酸法

以葡萄糖含量为指标

用L9(34)正交试验法选择最佳显色条件

检测波长为488nm。结果:苯酚、浓硫酸二因素对多糖含量有显著影响

以葡萄糖表示

本法在(3.223～12.89)μg.mL-1范围内

吸收度与浓度有良好的线性关系

平均回收率96.60%

RSD为1.71%。结论:本法快速、简便

灵敏度、重复性好

不受蛋白质干扰

亦可用于混有糖苷的粗多糖的测定。

Abstract

Objective:To establish UV method to determine polysaccharide in Fomitiporia robusta(P.Karst.) Fiasson.Method:The parameter of colorimetric method was optimiged through L9(34) orthogonal design with the index of glucose

by the use of phenyl hydrate-sulfuric acid

at the 488 nm.Result:The significant effects of phenyl hydrate-sulfuric acid volume on glucose content were discovered.The average of recovery was 96.6% and RSD 1.71% for glucose with in good linear relation in 3.223～12.89 μg·mL-1 with a correlation coefficient of 0.999 1.Conclution: The proposed method is sensitive

rapid and accurate.It can be used to assay polysaccharide with method indican.

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Related Author

LIU Minmin

LIU Ying

ZHANG Tao

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HUANG Min

XIE Yating

DENG Yaling

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Related Institution

Jiangxi Jingde Chinese Traditional Medicine Co. Ltd.

Jiangxi University of Chinese Medicine

School of Pharmacy， Chengdu University of Traditional Chinese Medicine，State Key Laboratory of Southwestern Chinese Medicine Resources， Key Laboratory of Standardization of Chinese Herbal Medicine，Ministry of Education

Shaanxi University of Chinese Medicine

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

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