etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC

ZHANG Ke-wei; WU Hao; CUI Xiao-bing; WANG Mei; SONG Shen

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etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC

更新时间：2024-01-04

- etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 10, Pages: 8-10(2008)
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- Published：2008
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ZHANG Ke-wei, WU Hao, CUI Xiao-bing, et al. etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 8-10.
DOI：

ZHANG Ke-wei, WU Hao, CUI Xiao-bing, et al. etermination of Geniposide and Liquiritigenin in Legan-Qingyan Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(10): 8-10. DOI：

摘要

目的:研究以HPLC法同时测定乐肝清炎胶囊中栀子苷、甘草素含量的方法。方法:采用KromasilC18柱(4.6mm×250mm

5μm)

以甲醇-0.5%冰乙酸为流动相

梯度洗脱

体积流量:1.0mL·min-1

λ1=238nm(检栀子苷)

λ2=276nm(检甘草素)

柱温30℃。结果:栀子苷在(54.1～162.4)μg·mL-1与峰面积呈良好的线性关系

r=0.9998

甘草素在(5.2～15.5)μg·mL-1与峰面积呈良好的线性关系

r=0.9999。平均加样回收率(n=9):栀子苷为99.66%;甘草素为99.90%。结论:本法操作简便

结果可靠

重现性好

可作为该产品质量控制的方法。

Abstract

Objective:To establish a HPLC method for determination of geniposide and liquiritigenin in Legan-Qingyan Capsules.Methods: A Kromasil C18 Column(4.6 mm×250 mm

5 μm)was used.The mobile phase was methanol-0.5% glacial acetic acid

the flow rate was 1.0 mL·min-1

λ1=238 nm(for the geniposide)

λ2=276 nm(for the liquiritigenin)

the column temperature was 30 ℃.Results: The linear range for geniposide was within(54.1～162.4)μg·mL-1

r=0.999 8

recovery was 99.66%(n=9).The linear range for liquiritigenin was within(5.2～15.5)μg·mL-1

r=0.999 9

recovery was 99.90%(n=9).Conclusion:This method has good reproducibility.It can be used for quality control in production of Legan-Qingyan Capsules.

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