Objective:To determine the contents of aloeemodin

rhein

emodin and chrysophanol in Qingyi Tablets by HPLC.Method: C18 column was used with mobile phase consisted of methanol-0.1%.Perchloric acid solution(75∶25)

at a flow rate of 1.0 mL·min-1.The detecting wavelength was set at 254nm.Result: The linearity of this method was good in the ranges of (0.062 4～0.312)μg for aloeemodin(r=0.999 9);(.156～0.780 μg for rhein(r=0.999 8);(0.062 4～0.312)μg for emodin(r=0.999 2);(0.156～0.780) μg for chrysophanol(r=0.999 9).The recoveries of aloeemodin

rhein

emoclin and chrysophanol were 99.31%

99.65%

99.10% and 99.51%.RSD were 1.71%

0.56%

1.82% and 1.24%

respectively.Conclusion: The method is accurate

precise and is suitable for assaysment of aloeemodin

rhein

emodin and chrysophanol in Qingyi Tablets.