Identifier and Determination of Chrysin in San-Ye-Chongcha

XU Guang-ming1; PENG Xin-jun1; XIAO Mei-feng1; WEN Li-zhang2

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Identifier and Determination of Chrysin in San-Ye-Chongcha

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- Identifier and Determination of Chrysin in San-Ye-Chongcha
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 7, Pages: 12-14(2008)
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- Published：2008
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XU Guang-ming1, PENG Xin-jun1, XIAO Mei-feng1, et al. Identifier and Determination of Chrysin in San-Ye-Chongcha[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 12-14.
DOI：

XU Guang-ming1, PENG Xin-jun1, XIAO Mei-feng1, et al. Identifier and Determination of Chrysin in San-Ye-Chongcha[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 12-14. DOI：

摘要

目的:鉴定和测定三叶虫茶中的白杨素苷。方法:经丙酮提取、聚酰胺柱色谱分离、重结晶纯化

利用化学鉴别、UVI、R、LC-ESI/MS/MS对白杨素苷进行鉴定;HPLC定量测定

Gemini C18柱

流动相∶乙腈-水(30∶70)

流速:1.0mL·min-1

柱温:40℃

检测波长:270nm。结果:从三叶虫茶中提取分离得白杨素-7-o-葡萄糖苷晶体

HPLC归一化法测得其纯度达99.2%;在(2～20)μg·min-1范围内

线性回归方程为:Y=7.739×104X-6.0×103(r=0.9993)

三批三叶虫茶中白杨素苷的平均含量为2.77%

平均回收率为97.3%

RSD为1.3%(n=5)。结论:首次从三叶虫茶中分离纯化得到白杨素-7-葡萄糖苷

且用HPLC法测定了其含量

测定结果准确、重现性好。

Abstract

Objective:To identify and quantitatively determine chrysin-7-o-glucoside in Sanyechongcha.Method:The compunds were extracted with acetone and purified with polyamide column and recrystallization.The chemical structure was identified by chemical method

IR and ESI/LC-MS/MS.The quantitative analysis was performed on a Gemini C18 column with acetonitrile-water(30∶70)as the mobile phase at flow rate of 1.0 mL·min-1.The detection wavelengh was at 270 nm.Result:The main ingredient with a purity of 99.2% was identified as chrysin-7-o-glucoside.The linear equation was Y=7.739×104X-6.0×103 in the range of 2～20 μg·mL-1(r=0.999 3).The content of chrysin-7-o-glucoside in San-ye-Chong-cha was 2.77% and average recovery was 97.3%.Conclusion: Chrysin-7-o-glucoside in Sanyechongcha was first isolated and quantitatively determined by HPLC

which was a simple

reliable and accurate method.

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