The Effect of the Cooling Blood to Remove Blood Stasis Formula on Regulation of the Cell Apoptosis Gene Expression of FHF Rats
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The Effect of the Cooling Blood to Remove Blood Stasis Formula on Regulation of the Cell Apoptosis Gene Expression of FHF Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 15, Issue 3, Pages: 32-34(2009)
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Published:2009
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BI Lei, CHEN Wei-ping, LI Wei-na, et al. The Effect of the Cooling Blood to Remove Blood Stasis Formula on Regulation of the Cell Apoptosis Gene Expression of FHF Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(3): 32-34.
DOI:
BI Lei, CHEN Wei-ping, LI Wei-na, et al. The Effect of the Cooling Blood to Remove Blood Stasis Formula on Regulation of the Cell Apoptosis Gene Expression of FHF Rats[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(3): 32-34.DOI:
The Effect of the Cooling Blood to Remove Blood Stasis Formula on Regulation of the Cell Apoptosis Gene Expression of FHF Rats
Objective:To study the effect of the cooling blood to remove blood stasis formula on gene expression profiles of cell apoptosis in FHF rats by gene chip technology
and discuss the molecular mechanism of the formula in counteracting FHF(fulminant hepatic failure).Method:Reproduce FHF rats models by D-GalN 500 mL·kg-1 and LPS 4.8 μg·kg-1 through intraperitoneal injection.Detect the differential expression between model group and drug treatment group by apoptosis gene chip(Oligo Apoptosis Microarray
Super Array Company).Result:There were 37 down-regulated genes and 10 up-regulated genes in the model group compared with the normal group;there were 16 down-regulated genes
among which there are 13 apoptosis-induced genes
and 12 up-regulated genes in the medicine group compared with the model group.Conclusion:The formula can inhibit hepatocyte apoptosis by regulating apoptosis-related genes through down-regulating apoptosis-induced gene and slightly up-regulating apoptosis-inhibited genes.