Study on Optimizing the Technological Condition of Ethanol Percolating Extraction for Xiaoqinglong Patch by Orthogonal Design Method

PAN Ling; TAI Xiao-hua; HAN Jian-wei

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Study on Optimizing the Technological Condition of Ethanol Percolating Extraction for Xiaoqinglong Patch by Orthogonal Design Method

更新时间：2024-01-04

- Study on Optimizing the Technological Condition of Ethanol Percolating Extraction for Xiaoqinglong Patch by Orthogonal Design Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 11, Pages: 20-22(2008)
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- Published：2008
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PAN Ling, TAI Xiao-hua, HAN Jian-wei. Study on Optimizing the Technological Condition of Ethanol Percolating Extraction for Xiaoqinglong Patch by Orthogonal Design Method[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(11): 20-22.
DOI：

PAN Ling, TAI Xiao-hua, HAN Jian-wei. Study on Optimizing the Technological Condition of Ethanol Percolating Extraction for Xiaoqinglong Patch by Orthogonal Design Method[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(11): 20-22. DOI：

摘要

目的:优选小青龙贴膏的乙醇渗漉提取工艺条件。方法:以盐酸麻黄碱和芍药苷提取量为考察指标

用高效液相色谱法测定其含量

采用正交试验设计对提取条件优选。结果:优选工艺为:以55%乙醇浸泡时间12h后渗漉

溶剂用量10倍

渗漉速度30mL.·min-1.kg-1。结论:优选工艺所得盐酸麻黄碱和芍药苷含量较高

工艺稳定可行。

Abstract

Objective:To optimize the technological condition of Ethanol percolating for Xiaoqinglong Patch.Method: The content of Ephedrinehydrochloride and Paeoniflorin extracted

which were regarded as chemical markers

were assayed by HPLC.Furthermore basis of initial research

the general content data determined were used to optimize the percolating extraction processing conditions by orthogonal Sampling design.Result: The optimum extraction condition was that the medicinal materials was soaked with 55% alcohol for 12h

then was percolated with 10 times amount of 55% alcohol and the percolating speed was 30 mL·min-1·kg-1.Conclusion: The content of Ephedrinehydrochloride and Paeoniflorin extracted by the the extraction process

were highest

and the optimiazed process is stable and efficient.

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