Application of Polyamide Resin in Purification of Hyperoside from  L.

KE Zhong-cheng1; GUI Shuang-ying2; ZHOU Ya-qiu2

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Application of Polyamide Resin in Purification of Hyperoside from L.

更新时间：2024-01-04

- Application of Polyamide Resin in Purification of Hyperoside from L.
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 11, Pages: 9-11(2008)
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- Published：2008
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KE Zhong-cheng1, GUI Shuang-ying2, ZHOU Ya-qiu2. Application of Polyamide Resin in Purification of Hyperoside from L.[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(11): 9-11.
DOI：

KE Zhong-cheng1, GUI Shuang-ying2, ZHOU Ya-qiu2. Application of Polyamide Resin in Purification of Hyperoside from L.[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(11): 9-11. DOI：

摘要

目的:研究聚酰胺树脂纯化贯叶金丝桃中金丝桃苷的工艺条件。方法:采用HPLC法测定金丝桃苷的含量;单因素法考察聚酰胺树脂对金丝桃苷的吸附性能以及洗脱参数。结果:聚酰胺树脂对提取液中金丝桃苷的最佳分离条件为:上柱液浓度为1.2mg·mL-1、流速为3.6BV·h-1

金丝桃苷比吸附量达2.33mg·g-1

金丝桃苷纯度可达28.96%。结论:聚酰胺树脂可用于贯叶金丝桃中金丝桃苷的分离富集。

Abstract

Objective:To study the technological parameters of the purification process of hyperoside from Hypericum perforatum extrative.Methods: HPLC was used to analyze the content of hyperoside in the samples from the different technological process

and the adsorption and elution parameters were investigated.Results: The optimal parameters were as follows:the sample extract solution

whose content of hyperoside was 1.2 mg·mL-1

was passed through the packed column with a flow rate of 3.6 BV·h-1.The adsorption rate of hyperoside was 2.33 mg·g-1

and the purity of hyperoside in the eluate reached 28.96%.Conclusion: Polyamide resin can be used to purify hyperoside in the extraction of Hypericum perforatum L.

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Related Author

PAN Jun1

YU Qing-hua1

GUI Shuang-ying 1

LIU Jing-biao1

DAI Min 1

Miao-kai LYU

Zhi-peng HUO

Related Institution

Development Center of Modern Chinese Medicine,Research Institute of Tasly Holding Group Co.Ltd.

State Key Laboratory of Critical Technology in Innovative Chinese Medicine,Tasly Pharmaceutical Group Co.Ltd.

School of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine

School of Pharmaceutical Science and Technology,Tianjin University

School of Pharmacy,China Pharmaceutical University

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