Influence of Clyster No.1 on the Express of Immunologic Targets of Mice with Ulcerative Colitis

LIU Ping1; AN Xiao-xia2; LI Yan2; DONG Bo2; SUN Shu-hua2; SHEN Xu-ni2; MAO Jian-ping2; CUI Yu-fang2

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Influence of Clyster No.1 on the Express of Immunologic Targets of Mice with Ulcerative Colitis

更新时间：2024-01-04

- Influence of Clyster No.1 on the Express of Immunologic Targets of Mice with Ulcerative Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 7, Pages: 63-66(2008)
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- Published：2008
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LIU Ping1, AN Xiao-xia2, LI Yan2, et al. Influence of Clyster No.1 on the Express of Immunologic Targets of Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 63-66.
DOI：

LIU Ping1, AN Xiao-xia2, LI Yan2, et al. Influence of Clyster No.1 on the Express of Immunologic Targets of Mice with Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(7): 63-66. DOI：

摘要

目的:比较中药方灌肠Ⅰ号与西药柳氮磺胺吡啶(SASP)对2

4-二硝基氯苯(DNCB)和乙酸(AA)复合法诱导的小鼠溃疡性结肠炎(UC)模型的治疗效果

并探寻UC发病机制。方法:将小鼠随机分为正常组、模型组、SASP组及灌肠Ⅰ号治疗组4组

用肠道积分法评定组织学变化;用流式细胞仪检测各组外周血、肠系膜淋巴结中CD3+、CD4+T细胞的变化。结果:模型组小鼠肠道组织学评分明显高于正常组

而结肠长度明显较正常组缩短;模型组CD3+、CD4+细胞较正常组明显降低(P<0.01)。经SASP和灌肠Ⅰ号灌肠治疗后

在肠道炎症和病理损伤明显减轻的同时

肠道组织学评分较模型组明显降低(P<0.01)

结肠长度与正常组基本接近

且CD3+、CD4+细胞亚群较模型组明显增高(P<0.01)。结论:灌肠Ⅰ号对DNCB-AA诱导的小鼠UC模型治疗取得了满意效果

可作为UC发病机制的研究及治疗的较理想药物。

Abstract

Objective:To compare the therapeutic effects of Clyster No.1(CN1

the Pulsatillae decoction) with salazosulfapyridine(SASP) to the ulcerative colitis(UC) model induced by dinitrochlorobenzene (DNCB)-acetic acid(AA) in mice

to research the pathogenesis.Methods:KM mice were randomly divided into four groups: normal control group

experimental colitis model group

SASP group and CN1 therapeutic group.Proportions of CD3+、CD4+ T cell、 in mesenteric lymph node and peripheral blood of each group were estimated by flow cytometry;and histological changes were evaluated by HE staining and histological score of intestine.Results: Compared with normal control group

the histological score was markedly higher and the length of colon became shorter in model group of experimental colitis.The proportion of CD3+、CD4+ T cells were markedly decreased(P<0.01) in model group compared with the control group.After two weeks' treatment with SASP and CN1

the histological score was reduced

the length of colon recovered almost to normal level

accompanied with increasing in the proportion of CD3+、CD4+ T cells compared with the model group.Conclusions: The effect was satisfactory for the UC mice treated with CN1 and it may serve as a tool for investigation of the pathogenesis and therapeutical effect for UC.

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Related Institution

School of Pharmacy，Anhui University of Chinese Medicine

Institute of Pharmaceutics，Anhui Academy of Chinese Medicine

Anhui Provincial Key Laboratory of Research & Development of Chinese Medicine

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