Determination of Total Flavonoids in Gubaoxinshen Tablets by UV Spectrophotometry

ZHANG Yan1; JIN Xiang-qun1; SHI Ying2

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Determination of Total Flavonoids in Gubaoxinshen Tablets by UV Spectrophotometry

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- Determination of Total Flavonoids in Gubaoxinshen Tablets by UV Spectrophotometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 3, Pages: 17-18(2009)
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- Published：2009
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ZHANG Yan1, JIN Xiang-qun1, SHI Ying2. Determination of Total Flavonoids in Gubaoxinshen Tablets by UV Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(3): 17-18.
DOI：

ZHANG Yan1, JIN Xiang-qun1, SHI Ying2. Determination of Total Flavonoids in Gubaoxinshen Tablets by UV Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(3): 17-18. DOI：

摘要

目的:测定骨宝昕肾片中总黄酮的含量

从而对本品质量进行控制。方法:采用紫外分光光度法测定

测定波长为270nm。结果:淫羊藿苷在2.55～20.44μg·mL-1的范围内

线性关系良好

回归方程为Y=0.037359X+0.000443

Abstract

Objective:To determin the total Flavonoids in Gubaoxinshen Tablets and to control the quality of the preparation.Method: The contents of total flavonoids were determined by UV spectrophotometry with a detection wavelength at 270 nm.Results: The calibration curve was linear in the range of 2.55～20.44 μg·mL-1.The regression equation was Y=0.037 359X+0.000 443(r=0.999 9) for icarrin.The average recovery was 99.2%

and RSD was 0.97%.Conclusion:The method is simple and accurate for the determination of the total flavonoids in Gubaoxinshen Tablets

and can be used as one of the quality control methods for the Gubaoxinshen Tablets.

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XU Ling-yuan

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Related Institution

First Hospital of Peking University

Weifang Institute for Drug Control

Shandong Academy of Chinese Medicine

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

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