Determination of Gallic Acid and Quercetin in Relinqing Preparations by HPLC

LI Yan-fang1; XIA Quan2; XU Feng-qing1; LIU Jin-qi1

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Determination of Gallic Acid and Quercetin in Relinqing Preparations by HPLC

更新时间：2024-01-04

- Determination of Gallic Acid and Quercetin in Relinqing Preparations by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 12, Pages: 15-17(2008)
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- Published：2008
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LI Yan-fang1, XIA Quan2, XU Feng-qing1, et al. Determination of Gallic Acid and Quercetin in Relinqing Preparations by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(12): 15-17.
DOI：

LI Yan-fang1, XIA Quan2, XU Feng-qing1, et al. Determination of Gallic Acid and Quercetin in Relinqing Preparations by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(12): 15-17. DOI：

摘要

目的:采用HPLC法同时测定热淋清制剂中没食子酸和槲皮素的含量。方法:采用KromosilC18色谱柱(4.6mm×250mm

5μm)

以乙腈-0.2%磷酸溶液梯度洗脱

流速为1mL·min-1

检测波长为254nm。结果:没食子酸在0.204～1.22μg(r=0.9997)

槲皮素在0.058～0.35μg(r=0.9995)范围内呈良好的线性关系;没食子酸回收率为98.5%

RSD为1.4%

槲皮素回收率为97.8%

RSD为1.6%。结论:HPLC法简便可行、重复性好

适用于热淋清制剂两种有效成分的同时测定

可用于热淋清制剂的质量控制。

Abstract

Objective:To develop an HPLC method for the determination of gallic acid and quercetin in Relinqing preparations.Method: A Kromosil C18 column(4.6 mm×250 mm

5 μm)was used

and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution. The flow rate was 1 mL·min-1 and the detection wavelength was 254 nm.Results:The linear ranges of gallic acid and quercetin were 0.204～1.22 μg(r=0.999 7)

0.058～0.35 μg(r=0.999 5) respectively.The average recoveries were 98.5%(RSD=1.4%)

97.8%(RSD=1.6%) respectively.Conclusion:The method can be used to control the quality of Relinqing preparations.It is simple

accurate

reproducible and suitable for simultaneous determination of two active constituents in Relinqing preparations.

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Related Institution

Department of Pharmacy,First Affiliated Hospital of Dalian Medical University

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Gansu Provincial Institute of Food and Drug Control,Gansu Province Traditional Chinese Medicine Quality and Safety Evaluation of Engineering Technology Research Center

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