Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro

您当前的位置：

首页 >

文章列表页 >

Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro

更新时间：2024-01-04

- Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 14, Issue 9, Pages: 47-50(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2008
- 稿件说明：
移动端阅览
Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 47-50.
DOI：

Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 47-50. DOI：

摘要

目的:探讨贞芪酪蛋白复合肽对Bel-7402人肝癌细胞分化的诱导作用

及对其端粒酶活性的影响。方法:贞芪酪蛋白复合肽给正常大鼠灌胃

采血制备药物血清。以正常鼠血清为阴性对照

维甲酸(RA)为阳性对照

将药物血清温育Bel-7402人肝癌细胞。噻唑蓝(MTT)比色法测定细胞增殖

图像分析系统测定细胞核质比

放射免疫法测定甲胎蛋白(AFP)含量

动态比色法测定γ-谷氨酰转肽酶(-γGT)和碱性磷酸酶(ALP)活性

多聚酶链反应—酶联免疫吸附法(PCR-ELISA法)检测细胞端粒酶活性。结果:贞芪酪蛋白复合肽药物血清可抑制人肝癌细胞的增殖

显著降低细胞核质比

抑制细胞分泌AFP

降低细胞-γGT活性

升高ALP活性

并有显著抑制人肝癌细胞端粒酶活性的作用。结论:贞芪酪蛋白复合肽具有诱导人肝癌细胞分化的作用

其主要机理可能是抑制了Bel-7402细胞端粒酶活性。

Abstract

Objective: To study the effect of ligustri-astragali casein co-peptid(LACP)drug serum in inducing differentiation of Bel-7402 human hepatocarcinoma cell(HHC) and its influence of the telomerase activity.Methods: Rats were fed with LACP decoction to prepare the drug-serum of LACP

and the drug-serum was used for Bel-7402 HHC culture.Serum of normal rat

was taken as negative control and that of retinoic acid(RA) as the positive control.Cell proliferation was detected by MTT colorimetric assay

nucleocytoplasm ratio by image assay

α-fetoprotein(AFP) by RIA

alkaline phosphatase(ALP) activity and γ-glutamyl transpeptidase(γ-GT) by dynamic colorimetric assay and telomerase activity of Bel-7402 cell by PCR-ELISA.Results: Drug-serum of FACP could inhibit the proliferation of Bel-7402 cell

markedly reduce the nucleocytoplasm ratio and the secretion of AFP

decrease γ-GT activity

increase ALP activity and suppress the telomerase activity of Bel-7402 cell.Conclusion: LACP can induce the differentiation of Bel-7402 HHC

the main mechanism maybe throngh the suppression of telomerase activity.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Correlation Between Telomere and Cardiovascular Aging and Traditional Chinese Medicine Intervention

Mechanism of Liuwei Dihuangwan in Enhancing Hepatocarcinoma Suicide Gene Therapy from Cx32

Study on Antitumor Action of Xiaoliu Tang in Mice

Ligustri-Astragali Casein Co-Peptide Serum Inhibition of Human Lung Adenocarcinoma A549 Cells and its Mechanicsm Study

Effects of Siteng Fang on Proliferation and Apoptosis in Bel-7402 Cells

Related Author

Xi-jie LI

Lian DUAN

Jie WANG

Jun-fang SU

Xue LI

Shao-feng WU

Ting-xiu ZHAO

Qiao LI

Related Institution

The First Affiliated Hospital of Yunnan University of Chinese Medicine

Guang'anmen Hospital, China Academy of Chinese Medical Sciences

School of Basic Medicine, School of Chinese Materia Medica, Guangzhou University of Chinese Medicine

The Third Affiliated Hospital,Sun Yat-Sen University

Gansu College of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰